Events

Date: 6 May 2026   Time: 15:00 - 16:00     

Location: GO Jones 610

Luis Bibiano will give a seminar: Particle characterization through super-resolution spectral microscopy - abstract below:
Spectroscopic Single Molecule Localization Microscopy (sSMLM) is a far-field spectroscopic imaging technique which is capable of simultaneously capturing the full emission spectrum of individual nanoparticles. In sSMLM, by incorporating a dispersive optical component, such as a grating, emission light from the sample is split into two imaging paths, spatial image representing the spatial distribution of blinking events and a spectral image containing the corresponding emission spectra.
By capturing and analysing unique spectroscopic signatures of quantum dots (QD) and Magic Size Clusters (MSC) we can recover the particle size distribution of nanoparticles. We demonstrate that the proposed spectral super-resolution method can be relatively easily implemented on any fluorescence microscopes equipped with appropriate light source, while offering fast image acquisition. Using this approach, we show that particle size distribution of MSCs is comparable to that of organic fluorophores, suggesting their monodisperse nature.
We show, the proposed methodology (that includes a corresponding data analysis routine) provides an alternative to the otherwise elaborate direct analysis of particle size distribution using TEM or other indirect methods (e.g. optical absorption or dynamic lights scattering).