Prof Steffi Krause
Professor of Electroanalytical Systems
School of Engineering and Materials Science
Queen Mary University of London
Queen Mary University of London
Research
Photoelectrochemical imaging, Light-addressable potentiometric sensors, Light-activated electrochemistry, Biosensors, Live-cell imaging
Interests
My research focuses on the development of chemical and biological sensors and electrochemical imaging.Light-Addressable Potentiometric Sensors (LAPS) and Scanning Photo-induced Impedance Microscopy (SPIM) are imaging techniques based on photocurrent measurements at electrolyte/insulator/silicon (EIS) field-effect structures. They are capable of measuring local changes in the surface potential (LAPS), which can be used to image ion concentrations, and of measuring the local impedance (SPIM). Recent highlights of our research are the achievement of submicron resolution of photocurrent measurements at silicon-on-sapphire substrates using a two-photon effect, the increase in the sensitivity of SPIM using organic, self-assembled monolayers as insulators, the use of SPIM for the interrogation of biosensor arrays for the detection of charged molecules such as DNA, quantitative impedance imaging of polyelectrolyte microcapsules with high resolution and the measurement of cell surface charge and cell impedance.
Recent development of photoelectrochemical imaging with semiconducting metal oxide substrates such as indium tin oxide and hematite allows detection of redox active molecules and hence a much broader range of analytes than with traditional EIS structures. Combined with a recently developed fast imaging setup, the technique has shown great potential for continuous functional electrochemical imaging of the attachment area of cells, providing information such as ion concentration, action potentials and cell membrane permeability. Photoelectrochemical imaging is particularly attractive for label-free in-situ monitoring in in-vitro models.
Publications
Publications of specific relevance to the Centre for Bioengineering2023
Gorecki J and Krause S (2023). Numerical investigation of a graphene-on-semiconductor device for optical monitoring of cell electrophysiology. iScience, Elsevier vol. 27 (1) 24-11-2023
2022
Jacques R, Zhou B, Marhuenda E, Gorecki J, Das A, Iskratsch T and Krause S (2022). Photoelectrochemical imaging of single cardiomyocytes and monitoring of their action potentials through contact force manipulation of organoids. Biosensors and Bioelectronics, Elsevier 21-12-2022
Krause S (2022). High-resolution impedance and potential imaging using light-addressable potentiometric sensors for bio-imaging applications. 23-11-2022
Zhou B, Jiang Y, Guo Q, Das A, Jorge Sobrido A, Hing K, Zayats A and Krause S (2022). Photoelectrochemical Detection of Calcium Ions Based on Hematite Nanorod Sensors. ACS Applied Nano Materials, American Chemical Society 10-11-2022
Meng Y, Chen F, Wu C, Krause S, Wang J and Zhang DW (2022). Light-Addressable Electrochemical Sensors toward Spatially Resolved Biosensing and Imaging Applications. ACS Sensors, American Chemical Society 28-06-2022
2021
Wan K, Liu Y, Santagiuliana G, Barandun G, Taroni Junior P, Güder F, Bastiaansen CWM, Baxendale M, Fenwick O, Papageorgiou DG, Krause S, Zhang H and Bilotti E (2021). Self-powered ultrasensitive and highly stretchable temperature–strain sensing composite yarns. Materials Horizons 12-07-2021
Zhou B, Das A, Zhong M, Guo Q, Zhang D-W, Hing KA, Sobrido AJ, Titirici M-M and Krause S (2021). Photoelectrochemical imaging system with high spatiotemporal resolution for visualizing dynamic cellular responses. Biosensors and Bioelectronics, Elsevier vol. 180 02-03-2021
2020
Tu Y, Kyle C, Luo H, Zhang DW, Das A, Briscoe J, Dunn S, Titirici MM and Krause S (2020). Ammonia Gas Sensor Response of a Vertical Zinc Oxide Nanorod-Gold Junction Diode at Room Temperature. ACS Sensors vol. 5 (11), 3568-3575. 25-11-2020
2019
Zhou B, Das A, Kappers M, Oliver R, Humphreys C and Krause S (2019). InGaN as a substrate for AC photoelectrochemical imaging. Sensors, MDPI 11-10-2019
Wang J, Yang Z, Chen W, Du L, Jiao B, Krause S, Wang P, Wei Q, Zhang D-W and Wu C (2019). Modulated light-activated electrochemistry at silicon functionalized with metal-organic frameworks towards addressable DNA chips. Biosensors and Bioelectronics, Elsevier vol. 146, 111750-111750. 03-10-2019
Wan K, Taroni PJ, Liu Z, Liu Y, Tu Y, Santagiuliana G, Hsia I, Zhang H, Fenwick O, Krause S, Baxendale M, Schroeder BC and Bilotti E (2019). Flexible and Stretchable Self‐Powered Multi‐Sensors Based on the N‐Type Thermoelectric Response of Polyurethane/Na
x
(Ni‐ett)
n
Composites. Advanced Electronic Materials, Wiley, 1900582-1900582. 15-09-2019
Ahmad N, Colak B, Gibbs MJ, Zhang D-W, Gautrot JE, Watkinson M, Becer CR and Krause S (2019). Peptide Cross-Linked Poly(2-oxazoline) as a Sensor Material for the Detection of Proteases with a Quartz Crystal Microbalance. Biomacromolecules vol. 20 (7), 2506-2514. 07-06-2019
Wu F, Zhou B, Wang J, Zhong M, Das A, Watkinson M, Hing K, Zhang D-W and Krause S (2019). Photoelectrochemical Imaging System for the Mapping of Cell Surface Charges. Anal Chem 15-04-2019
Ahmad N, Colak B, Zhang D-W, Gibbs MJ, Watkinson M, Becer CR, Gautrot JE and Krause S (2019). Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase. Sensors, MDPI vol. 19 (7) 08-04-2019
2018
Zhang D, Papaioannou N, Titirici M-M and Krause S (2018). Photoelectrochemical Imaging Using Carbon Dots (CDs) Derived from Chitosan. Proceedings, MDPI vol. 2 (13) 10-12-2018
Ahmad N, Colak B, Gibbs MJ, Zhang D-W, Becer CR, Watkinson M, Gautrot JE and Krause S (2018). Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films. 30-11-2018
Tu Y, Li J, Zhang D-W, Briscoe J and Krause S (2018). The Effect of Semiconductor Morphology on the Spatial Resolution of ZnO Based Light-Addressable Potentiometric Sensors. EUROSENSORS 2018. 27-11-2018
TU Y, AHMAD N, BRISCOE J, ZHANG D and KRAUSE S (2018). Light-Addressable Potentiometric Sensors using ZnO Nanorods as the Sensor Substrate for Bioanalytical Applications. Analytical Chemistry, American Chemical Society 22-06-2018
Wang J, Du L, Krause S, Wu C and Wang P (2018). Surface modification and construction of LAPS towards biosensing applications. Sensors and Actuators, B: Chemical vol. 265, 161-173. 08-03-2018
ZHANG D, PAPAIOANNOU N, David NM, LUO H, GAO H, Tanase LC, Degousee T, Samori P, SAPELKIN A, FENWICK O, TITIRICI M and KRAUSE SK (2018). Photoelectrochemical response of carbon dots (CDs) derived from chitosan and their use in electrochemical imaging. Materials Horizons, Rsc 12-02-2018
TU Y, CHEN S, LI X, GORBACIOVA J, GILLIN WP, KRAUSE S and BRISCOE J (2018). Control of oxygen vacancies in ZnO nanorods by annealing and their influence on ZnO/PEDOT:PSS diode behaviour. Journal of Materials Chemistry C, Royal Society of Chemistry 02-02-2018
Schöning MJ, Wagner T, Poghossian A, Miyamoto K-I, Werner CF, Krause S and Yoshinobu T (2018). Light-Addressable Potentiometric Sensors for (Bio-)chemical Sensing and Imaging. Encyclopedia of Interfacial Chemistry 295-308. 01-01-2018
Yoshinobu T, Krause S, Miyamoto K-I, Werner CF, Poghossian A, Wagner T and Schöning MJ (2018). (Bio-)chemical Sensing and Imaging by LAPS and SPIM. Label-Free Biosensing 103-132. 01-01-2018
2017
Zhang D, Wu F and KRAUSE SK (2017). LAPS and SPIM imaging using ITO coated glass as the substrate material. Analytical Chemistry, American Chemical Society 05-07-2017
Wu F, Campos I, Zhang D and KRAUSE SK (2017). Biological imaging using light-addressable potentiometric sensors and scanning photo-induced impedance microscopy. Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences, Royal Society, The 03-05-2017
Zhang D and Krause S (2017). Image Detection of Yeast Saccharomyces Cerevisiae By Light-Addressable Potentiometric Sensors (LAPS). ECS Meeting Abstracts, The Electrochemical Society vol. MA2017-01 (38), 1770-1770. 15-04-2017
Wu F, Watkinson M and Krause S (2017). Electrochemical Imaging of Copper Contamination on Self-Assembled Organic Monolayer Modified Silicon Surfaces Following a ‘Click’ Reaction with Light-Addressable Potentiometric Sensors and Scanning Photo-Induced Impedance Microscopy. ECS Meeting Abstracts, The Electrochemical Society vol. MA2017-01 (38), 1769-1769. 15-04-2017
Wu F, Zhang DW, Wang J, Watkinson M and Krause S (2017). Copper Contamination of Self-Assembled Organic Monolayer Modified Silicon Surfaces Following a “Click” Reaction Characterized with LAPS and SPIM. Langmuir vol. 33 (13), 3170-3177. 04-04-2017
2016
Zhang DW, Wu F, Wang J, Watkinson M and KRAUSE SK (2016). Image detection of yeast Saccharomyces cerevisiae by light-addressable potentiometric sensors (LAPS). Electrochemistry Communications vol. 72, 41-45. 24-08-2016
Wang J, Campos I, Wu F, Zhu J, Sukhorukov GB, Palma M, Watkinson M and Krause S (2016). The effect of gold nanoparticles on the impedance of microcapsules visualized by scanning photo-induced impedance microscopy. Electrochimica Acta vol. 208, 39-46. 04-05-2016
2015
Wang J, Wu F, Watkinson M, Zhu J and Krause S (2015). click Patterning of Self-Assembled Monolayers on Hydrogen-Terminated Silicon Surfaces and Their Characterization Using Light-Addressable Potentiometric Sensors. Langmuir vol. 31 (35), 9646-9654. 08-09-2015
Gibbs MJ, Biela A and Krause S (2015). α-Amylase sensor based on the degradation of oligosaccharide hydrogel films monitored with a quartz crystal sensor. Biosens Bioelectron vol. 67, 540-545. 15-05-2015
Zhang H, Yilmaz P, Ansari JO, Khan FF, Binions R, Krause S and Dunn S (2015). Incorporation of Ag nanowires in CuWO4 for improved visible light-induced photoanode performance. Journal of Materials Chemistry A vol. 3 (18), 9638-9644. 26-03-2015
Biela A, Watkinson M, Meier UC, Baker D, Giovannoni G, Becer CR and Krause S (2015). Disposable MMP-9 sensor based on the degradation of peptide cross-linked hydrogel films using electrochemical impedance spectroscopy. Biosensors and Bioelectronics vol. 68, 660-667. 27-01-2015
2014
Wang J, Zhou Y, Watkinson M, Gautrot J and Krause S (2014). High-sensitivity light-addressable potentiometric sensors using silicon on sapphire functionalized with self-assembled organic monolayers. Sensors and Actuators B: Chemical, Elsevier vol. 209, 230-236. 26-11-2014
2013
Dzaraini K, Norizan A, Krause S, Hunter C and Milanesi L (2013). Degradation behaviour of thin polymer films of poly (amide ester) hydrogel using quartz crystal microbalance. Advanced Materials Research vol. 812, 38-45. 29-10-2013
2012
Zhang DW, Zhang FT, Cui YR, Deng QP, Krause S, Zhou YL and Zhang XX (2012). A label-free aptasensor for the sensitive and specific detection of cocaine using supramolecular aptamer fragments/target complex by electrochemical impedance spectroscopy. Talanta vol. 92, 65-71. 15-04-2012
2011
Zheng X, Cook JP, Watkinson M, Yang S, Douglas I, Rawlinson A and Krause S (2011). Generic protease detection technology for monitoring periodontal disease. Faraday Discussions vol. 149, 37-47. 17-01-2011
2010
Chen L, Zhou Y, Jiang S, Kunze J, Schmuki P and Krause S (2010). High resolution LAPS and SPIM. Electrochemistry Communications vol. 12 (6), 758-760. 01-06-2010
2009
Kamarun D, Zheng XW, Milanesi L, Hunter CA and Krause S (2009). A peptide cross-linked polyacrylamide hydrogel for the detection of human neutrophil elastase. ELECTROCHIMICA ACTA vol. 54 (22), 4985-4990. 01-09-2009
Stair JL, Watkinson M and Krause S (2009). Sensor materials for the detection of proteases. Biosensors and Bioelectronics vol. 24 (7), 2113-2118. 15-03-2009
Sweeney SJ and Krause S (2009). Selected Papers from the Second International Conference on Optical, Optoelectronic and Photonic Materials and Applications, 2007. J MATER SCI-MATER EL vol. 20, 1-2. 01-01-2009
Sweeney SJ and Krause S (2009). Editorial: Selected papers from the second international conference on optical, optoelectronic and photonic materials and applications, 2007. Journal of Materials Science: Materials in Electronics vol. 20 (SUPPL. 1) 01-01-2009
2008
Chen L, Zhou Y, Krause S, Munoz AG, Kunze J and Schmuki P (2008). Repair of thin thermally grown silicon dioxide by anodic oxidation. Electrochimica Acta vol. 53 (8), 3395-3402. 10-03-2008
2007
Zhou Y, Jiang S, Krause S and Chazalviel J-N (2007). Biosensor arrays based on the degradation of thin polymer films interrogated by scanning photoinduced impedance microscopy. Anal Chem vol. 79 (23), 8974-8978. 01-12-2007
Zhou Y, Chen L, Krause S and Chazalviel J-N (2007). Scanning photoinduced impedance microscopy using amorphous silicon photodiode structures. Anal Chem vol. 79 (16), 6208-6214. 15-08-2007
2006
Krause S, Moritz W, Talabani H, Xu M, Sabot A and Ensell G (2006). Scanning Photo-Induced Impedance Microscopy - Resolution studies and polymer characterization. ELECTROCHIMICA ACTA vol. 51 (8-9), 1423-1430. 20-01-2006
Zhou YL, Krause S, Chazalviel JN and McNeil CJ (2006). Biosensor arrays based on the degradation of thin polymer films interrogated by Scanning Photo-induced Impedance Microscopy. 01-01-2006
KRAUSE SK, Fernandez-Sanchez C and McNeil CJ (2006). Sensors based on thin film degradation. Encyclopedia of Sensors 289-306. 01-01-2006
2004
Moritz W, Yoshinobu T, Finger F, Krause S, Martin-Fernandez M and Schoning MJ (2004). High resolution LAPS using amorphous silicon as the semiconductor material. SENSORS AND ACTUATORS B-CHEMICAL vol. 103 (1-2), 436-441. 29-09-2004
2003
KRAUSE SK (2003). Impedance methods. Encyclopedia of Electrochemistry, Volume 3, Instrumentation and Electroanalytical Chemistry 196-229. 01-01-2003
2002
Sabot A and Krause S (2002). Simultaneous quartz crystal microbalance impedance and electrochemical impedance measurements. Investigation into the degradation of thin polymer films. Anal Chem vol. 74 (14), 3304-3311. 15-07-2002
Krause S, Talabani H, Xu M, Moritz W and Griffiths J (2002). Scanning photo-induced impedance microscopy - an impedance based imaging technique. ELECTROCHIMICA ACTA vol. 47 (13-14), 2143-2148. 25-05-2002
2001
Sumner C, Krause S, Sabot A, Turner K and McNeil CJ (2001). Biosensor based on enzyme-catalysed degradation of thin polymer films. BIOSENSORS & BIOELECTRONICS vol. 16 (9-12), 709-714. 01-12-2001
Moritz W, Krause S, Roth U, Klimm D and Lippitz A (2001). Re-activation of an all solid state oxygen sensor. ANAL CHIM ACTA vol. 437 (2), 183-190. 27-06-2001
KRAUSE SK, Sabot A and Sumner C (2001). Combined QCM and electrochemical impedance measurements for biosensor applications. Chemical and Biological Sensors and Analytical Methods II, ECS Conference, San Francisco. 01-01-2001
Wilkop T, Krause S, Nabok A, Ray AK and Yates R (2001). The detection of organic pollutants in water with calixarene coated electrodes., Editors: Moebius D and Miller R. 01-01-2001
2000
Sumner C, Sabot A, Turner K and Krause S (2000). A transducer based on enzyme-induced degradation of thin polymer films monitored by surface plasmon resonance. Anal Chem vol. 72 (21), 5225-5232. 01-11-2000
Moritz W, Gerhardt I, Roden D, Xu M and Krause S (2000). Photocurrent measurements for laterally resolved interface characterization. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY vol. 367 (4), 329-333. 01-06-2000
1999
Ho WO, Krause S, McNeil CJ, Pritchard JA, Armstrong RD, Athey D and Rawson K (1999). Electrochemical sensor for measurement of urea and creatinine in serum based on ac impedance measurement of enzyme-catalyzed polymer transformation. ANAL CHEM vol. 71 (10), 1940-1946. 15-05-1999
1998
Moritz W, Krause S, Bartholomäus L, Gabusjan T, Vasiliev AA, Godovski DY and Malyshev VV (1998). Silicon-Based Sensor for Flourine Gas. ACS Symposium Series vol. 690, 119-129. 01-01-1998
1995
McNeil CJ, Athey D, Ball M, Ho WO, Krause S, Armstrong RD, Des Wright J and Rawson K (1995). Electrochemical Sensors Based on Impedance Measurement of Enzyme-Catalyzed Polymer Dissolution: Theory and Applications. Analytical Chemistry, American Chemical Society (ACS) vol. 67 (21), 3928-3935. 01-11-1995
Moritz W, Krause S, Vasiliev AA, Godovski DY and Malyshev VV (1995). Monitoring of HF and F2 using a field-effect sensor. Sensors and Actuators B Chemical, Elsevier vol. 24 (1-3), 194-196. 01-03-1995
1994
Krause S, Moritz W and Grohmann I (1994). Improved long-term stability for an LaF3 based oxygen sensor. Sensors and Actuators B Chemical, Elsevier vol. 18 (1-3), 148-154. 01-03-1994
1992
Krause S, Moritz W and Grohmann I (1992). A low-temperature oxygen sensor based on the Si/LaF3/Pt capacitive structure. Sensors and Actuators B Chemical, Elsevier vol. 9 (3), 191-196. 01-10-1992
1991
Bobrov PV, Tarantov YA, Krause S and Moritz W (1991). Chemical sensitivity of an ISFET with Ta2O5 membrane in strong acid and alkaline solutions. Sensors and Actuators B Chemical, Elsevier vol. 3 (1), 75-81. 01-01-1991
Grants
Grants of specific relevance to the Centre for Bioengineering3D Photoelectrochemical Imaging in Porous Light-Addressable StructuresSteffi Krause, Joseph Briscoe and Thomas Iskratsch
£202,248 EPSRC Engineering and Physical Sciences Research Council (01-02-2021 - 30-04-2023)
Combined LAPS and SICM for multimodal live cell imagingSteffi Krause
£504,481 EPSRC Engineering and Physical Sciences Research Council (01-06-2018 - 28-02-2023)