Dr Ivan Kadurin
Lecturer in Physiology
School of Biological and Behavioural Sciences
Queen Mary University of London
Queen Mary University of London
Research
ion channels, neuronal excitation, electrophysiology, biochemistry, sub-cellular protein trafficking
Interests
Calcium influx triggered by electrical excitation, regulated by Voltage-Activated Calcium Channels, is the fast and ubiquitous chemical signal in excitable tissues like the brain, heart, and muscle. Our group investigates the structure-function relationship, interactions, and cellular trafficking of neuronal and cerebrovascular Calcium Channel proteins. We use a combination of electrophysiology, flow cytometry, immunocytochemistry and biochemical techniques to investigate how the assembly of different protein subunits regulates the channels' biological activity and the mechanisms of action of drugs targeting them. We use both heterologous cell expression systems and primary neurons to investigate the channel function and cellular trafficking.Publications
2022
Kadurin I, Dahimene S, Page KM, Ellaway JIJ, Chaggar K, Troeberg L, Nagase H and Dolphin AC (2022). ADAM17 Mediates Proteolytic Maturation of Voltage-Gated Calcium Channel Auxiliary α2δ Subunits, and Enables Calcium Current Enhancement. Function, Oxford University Press (OUP) vol. 3 (3) 17-03-2022
Dahimene S, von Elsner L, Holling T, Mattas LS, Pickard J, Lessel D, Pilch KS, Kadurin I, Pratt WS, Zhulin IB, Dai H, Hempel M, Ruzhnikov MRZ, Kutsche K and Dolphin AC (2022). Biallelic CACNA2D1 loss-of-function variants cause early-onset developmental epileptic encephalopathy. Brain, Oxford University Press (OUP) vol. 145 (8), 2721-2729. 16-03-2022
2020
Dolphin AC and Kadurin I (2020). Fight or flight: The culprit is lurking in the neighbourhood. Cell Calcium, Elsevier vol. 87 11-02-2020
2019
Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS and Dolphin AC (2019). Disruption of the Key Ca2+ Binding Site in the Selectivity Filter of Neuronal Voltage-Gated Calcium Channels Inhibits Channel Trafficking. Cell Reports, Elsevier vol. 29 (1), 22-33.e5. 01-10-2019
2018
Dahimene S, Page KM, Kadurin I, Ferron L, Ho DY, Powell GT, Pratt WS, Wilson SW and Dolphin AC (2018). The α2δ-like Protein Cachd1 Increases N-type Calcium Currents and Cell Surface Expression and Competes with α2δ-1. Cell Reports, Elsevier vol. 25 (6), 1610-1621.e5. 01-11-2018
Ferron L, Kadurin I and Dolphin AC (2018). Proteolytic maturation of α2δ controls the probability of synaptic vesicular release. eLife, eLife vol. 7 19-06-2018
2017
Kadurin I, Rothwell SW, Lana B, Nieto-Rostro M and Dolphin AC (2017). LRP1 influences trafficking of N-type calcium channels via interaction with the auxiliary α2δ-1 subunit. Scientific Reports, Springer Nature vol. 7 (1) 03-03-2017
2016
Kadurin I, Ferron L, Rothwell SW, Meyer JO, Douglas LR, Bauer CS, Lana B, Margas W, Alexopoulos O, Nieto-Rostro M, Pratt WS and Dolphin AC (2016). Proteolytic maturation of α2δ represents a checkpoint for activation and neuronal trafficking of latent calcium channels. eLife, eLife vol. 5 26-10-2016
Lana B, Page KM, Kadurin I, Ho S, Nieto-Rostro M and Dolphin AC (2016). Thrombospondin-4 reduces binding affinity of [3H]-gabapentin to calcium-channel α2δ-1-subunit but does not interact with α2δ-1 on the cell-surface when co-expressed. Scientific Reports, Springer Nature vol. 6 (1) 14-04-2016
2014
Cassidy JS, Ferron L, Kadurin I, Pratt WS and Dolphin AC (2014). Functional exofacially tagged N-type calcium channels elucidate the interaction with auxiliary α2δ-1 subunits. Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences vol. 111 (24), 8979-8984. 02-06-2014
Alvarez-Laviada A, Kadurin I, Senatore A, Chiesa R and Dolphin AC (2014). The inhibition of functional expression of calcium channels by prion protein demonstrates competition with α2δ for GPI-anchoring pathways. Biochemical Journal, Portland Press vol. 458 (2), 365-374. 14-02-2014
2012
Kadurin I, Alvarez-Laviada A, Ng SFJ, Walker-Gray R, D'Arco M, Fadel MG, Pratt WS and Dolphin AC (2012). Calcium Currents Are Enhanced by α2δ-1 Lacking Its Membrane Anchor*. Journal of Biological Chemistry, Elsevier vol. 287 (40), 33554-33566. 06-08-2012
Kadurin I, Bauer C, Lana B, Alvarez-Laviada A, Nieto-Rostro M, Douglas L, Troeberg L, Nagase H and Dolphin AC (2012). Voltage-Gated Calcium Channel α2δ Subunits in Lipid Rafts: The Importance of Proteolytic Cleavage Into α2 and δ. 01-01-2012
2011
Huang Z, Lujan R, Kadurin I, Uebele VN, Renger JJ, Dolphin AC and Shah MM (2011). Presynaptic HCN1 channels regulate CaV3.2 activity and neurotransmission at select cortical synapses. Nature Neuroscience, Springer Nature vol. 14 (4), 478-486. 27-02-2011
2010
Bauer CS, Tran-Van-Minh A, Kadurin I and Dolphin AC (2010). A new look at calcium channel α2δ subunits. Current Opinion in Neurobiology, Elsevier vol. 20 (5), 563-571. 25-06-2010
Davies A, Kadurin I, Alvarez-Laviada A, Douglas L, Nieto-Rostro M, Bauer CS, Pratt WS and Dolphin AC (2010). The α2δ subunits of voltage-gated calcium channels form GPI-anchored proteins, a posttranslational modification essential for function. Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences vol. 107 (4), 1654-1659. 04-01-2010
2009
Bauer CS, Nieto-Rostro M, Rahman W, Tran-Van-Minh A, Ferron L, Douglas L, Kadurin I, Ranjan YS, Fernandez-Alacid L, Millar NS, Dickenson AH, Lujan R and Dolphin AC (2009). The Increased Trafficking of the Calcium Channel Subunit α2δ-1 to Presynaptic Terminals in Neuropathic Pain Is Inhibited by the α2δ Ligand Pregabalin. Journal of Neuroscience, Society for Neuroscience vol. 29 (13), 4076-4088. 01-04-2009
Kadurin I, Huber S and Gründer S (2009). A single conserved proline residue determines the membrane topology of stomatin. Biochemical Journal, Portland Press vol. 418 (3), 587-594. 25-02-2009
2008
Kadurin I, Golubovic A, Leisle L, Schindelin H and Gründer S (2008). Differential effects of N-glycans on surface expression suggest structural differences between the acid-sensing ion channel (ASIC) 1a and ASIC1b. Biochemical Journal, Portland Press vol. 412 (3), 469-475. 28-05-2008
2007
Chen X, Polleichtner G, Kadurin I and Gründer S (2007). Zebrafish Acid-sensing Ion Channel (ASIC) 4, Characterization of Homo- and Heteromeric Channels, and Identification of Regions Important for Activation by H+ *. Journal of Biological Chemistry, Elsevier vol. 282 (42), 30406-30413. 07-08-2007
2006
Chen X, Paukert M, Kadurin I, Pusch M and Gründer S (2006). Strong modulation by RFamide neuropeptides of the ASIC1b/3 heteromer in competition with extracellular calcium. Neuropharmacology, Elsevier vol. 50 (8), 964-974. 20-03-2006
Grants
Grants of specific relevance to the Centre for Molecular Cell BiologyAmyloids and Oligomers. Curvilinear and annular structures and their interation with exosomes and whole cellsJohn Viles, Ivan Kadurin, Vladimir Volkov and Vidya Darbari
£485,209 BBSRC Biotechnology and Biological Sciences Research Council (01-12-2023 - 30-11-2026)
Proteolytic activation of calcium channels: potential target for channel - directed therapeutics in arterial smooth muscle cells - Grant Transfer Dr I KadurinIvan Kadurin
£488,751 MRC Medical Research Council (01-11-2022 - 31-10-2025)
Highthroughput Light Sheet Microscopy: Imaging Across ScalesViji Draviam Sastry, Caroline Brennan, Vidya Darbari, Jose Martin Duran, Ivan Kadurin, Maria Isabel Palacios De Castro, Richard Pickersgill, Sarah McClelland, Tyson Sharp and Martin Knight
£386,205 MRC Medical Research Council (01-10-2022 - 31-03-2023)