Prof Richard Pickersgill

Publications

Publications of specific relevance to the Centre for Molecular Cell Biology

2024

Enzyme-cargo encapsulation peptides bind between tessellating tiles of the bacterial microcompartment shell
Gu S Bradley-Clarke J Rose R-S Warren MJ Pickersgill RW
Journal of Biological Chemistry, Elsevier vol. 300 (6) 

DOI 10.1016/j.jbc.2024.107357

QMRO

10-05-2024

2022

Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
Zhang S Gu S Rycroft P Ruaudel F Delolme F
Mbio, American Society For Microbiology, e0025322-e0025322.  

DOI 10.1128/mbio.00253-22

QMRO

12-05-2022

2020

Lig v 1 structure and the inflammatory response to the Ole e 1 protein family.
Robledo Retana T Bradley-Clarke J Croll T Rose R Stagg AJ Villalba M Honti I Pickersgill RW
Allergy 

DOI 10.1111/all.14351

QMRO

02-05-2020

2019

Kinetochores attached to microtubule-ends are stabilised by astrin bound pp1 to ensure proper chromosome segregation
Conti D Gul P Islam A Martín-Durán JM
Elife, Elife Sciences Publications Ltd vol. 8 

DOI 10.7554/eLife.49325

QMRO

24-12-2019

Structure of Nanotubes Formed From Bacterial Microcompartment Shell Proteins
UDDIN I PICKERSGILL R Gosal W
 

QMRO

10-01-2019

2018

De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments
Lee MJ Mantell J Brown IR Fletcher JM Verkade P Woolfson DN
Nature Communications, Springer Nature vol. 9 (1) 

DOI 10.1038/s41467-018-05922-x

QMRO

24-08-2018

Structural basis of pyrrole polymerization in human porphobilinogen deaminase.
Pluta P Roversi P Bernardo-Seisdedos G Rojas AL Gu S Pickersgill RW
Biochim Biophys Acta 

DOI 10.1016/j.bbagen.2018.06.013

QMRO

14-06-2018

A Generic Self-Assembly Process in Microcompartments and Synthetic Protein Nanotubes
Uddin I Frank S Pickersgill RW
Small vol. 14 (19) 

DOI 10.1002/smll.201704020

QMRO

09-05-2018

Synthetic Protein Nanotubes: A Generic Self‐Assembly Process in Microcompartments and Synthetic Protein Nanotubes (Small 19/2018)
Uddin I Frank S Warren MJ
Small, Wiley vol. 14 (19) 

DOI 10.1002/smll.201870087

01-05-2018

2017

Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes
Abbott W Alber O Bayer E Berrin J-G Boraston A Brumer H Brzezinski R Clarke A et al.
Glycobiology vol. 28 (1), 3-8.  

DOI 10.1093/glycob/cwx089

QMRO

11-10-2017

A review of drug therapy for sporadic fatal insomnia.
Tabaee Damavandi P Pickersgill RW
Prion vol. 11 (5), 293-299.  

DOI 10.1080/19336896.2017.1368937

QMRO

04-10-2017

The role of intrinsic disorder and dynamics in the assembly and function of the type II secretion system
Gu S Vladimir VE Pickersgill Garnett JA
Bba - Proteins and Proteomics, Elsevier 

DOI 10.1016/j.bbapap.2017.07.006

QMRO

19-07-2017

Introducing Quasi-Optical Terahertz Circular Dichroism Spectroscopy
Qui J Zeng Y Pickersgill R Sushko O
2017 XI International Conference on Antenna Theory and Techniques (ICATT)., 26-29.  

DOI 10.1109/icatt.2017.7972579

01-05-2017

2015

Crystal structure of CobK reveals strand-swapping between Rossmann-fold domains and molecular basis of the reduced precorrin product trap
Gu S Sushko O Deery E
Scientific Reports, Springer Nature vol. 5 (1) 

DOI 10.1038/srep16943

QMRO

30-11-2015

Structural insights into the loss of catalytic competence in pectate lyase activity at low pH
Ali S Teixeira S Pickersgill RW
Febs Letters, Wiley vol. 589 (21), 3242-3246.  

DOI 10.1016/j.febslet.2015.09.014

QMRO

28-09-2015

Sub-Terahertz Spectroscopy as a Probe for Protein Stability in an Ionic Environment
Sushko O Qiu J Dubrovka R
2015 40th International Conference on Infrared, Millimeter, and Terahertz waves (IRMMW-THz)., 1-2.  

DOI 10.1109/irmmw-thz.2015.7327768

01-08-2015

2014

Structural insights into higher order assembly and function of the bacterial microcompartment protein PduA.
Pang A Frank S Warren MJ
J Biol Chem vol. 289 (32), 22377-22384.  

DOI 10.1074/jbc.M114.569285

08-08-2014

FAD binding, cobinamide binding and active site communication in the corrin reductase (CobR).
Lawrence AD Taylor SL Scott A Rowe ML Johnson CM Rigby SEJ Geeves MA
Biosci Rep vol. 34 (4) 

DOI 10.1042/BSR20140060

04-07-2014

The structure, function and properties of sirohaem decarboxylase - an enzyme with structural homology to a transcription factor family that is part of the alternative haem biosynthesis pathway
Palmer DJ Schroeder S Deery E Lobo SA Saraiva LM McLean KJ Ferguson SJ Pickersgill RW et al.
Molecular Microbiology vol. 93 (2), 247-261.  

DOI 10.1111/mmi.12656

01-01-2014

2013

The effect of the adaptor protein Isd11 on the quaternary structure of the eukaryotic cysteine desulphurase Nfs1.
Terali K Beavil RL Pickersgill RW
Biochem Biophys Res Commun vol. 440 (2), 235-240.  

DOI 10.1016/j.bbrc.2013.09.039

18-10-2013

Crystal structure of the bacterial type II secretion system secretin/pilotin complex
Gu S Rehman S
Acta Crystallographica Section A: Foundations and advances. vol. 69 (a1), s351-s351.  

DOI 10.1107/s0108767313096979

25-08-2013

Anatomy of secretin binding to the Dickeya dadantii type II secretion system pilotin.
Rehman S Gu S Shevchik VE
Acta Crystallogr D Biol Crystallogr vol. 69 (Pt 8), 1381-1386.  

DOI 10.1107/S0907444913007658

QMRO

01-08-2013

Lagrangian dynamics analysis of sub-THz skeletal absorption spectra of amino acids acquired by vector network analysis
DONNAN RS Pickersgill R Krauss N
Chemical Physics Letters, Elsevier vol. 558, 104-108.  

DOI 10.1016/j.cplett.2012.12.044

12-02-2013

2012

Substrate channels revealed in the trimeric Lactobacillus reuteri bacterial microcompartment shell protein PduB.
Pang A Liang M Prentice MB Pickersgill RW
Acta Crystallogr D Biol Crystallogr vol. 68 (Pt 12), 1642-1652.  

DOI 10.1107/S0907444912039315

01-12-2012

A joint neutron/X-ray crystallographic study on the mechanism of pectate lyase
Ali S Blakeley M Søndergaard CR Pellegrini E
Acta Crystallographica Section A: Foundations and advances. vol. 68 (a1), s91-s91.  

DOI 10.1107/s0108767312098236

07-08-2012

Solution structure of homology region (HR) domain of type II secretion system.
Gu S Kelly G Wang X Shevchik VE
J Biol Chem vol. 287 (12), 9072-9080.  

DOI 10.1074/jbc.M111.300624

16-03-2012

Structural and functional insights into the pilotin-secretin complex of the type II secretion system.
Gu S Rehman S Wang X Shevchik VE
Plos Pathog vol. 8 (2) 

DOI 10.1371/journal.ppat.1002531

QMRO

01-02-2012

Pathology: A Structural Biologist's View
Pickersgill RW
JOURNAL OF PATHOLOGY. vol. 226, S7-S7.  
01-01-2012

Intestinal Glycogen Storage Disease, and the Molecular Basis of Pseudo-obstruction with Polyglucosan Bodies
Martin JE Chowdhury ATMD McElwaine S Fikree A Minassian B Broad J Knowles CH Allen L et al.
JOURNAL OF PATHOLOGY. vol. 226, S24-S24.  
01-01-2012

Cysteine Scanning Mutagenesis and Disulfide Mapping Analysis of Arrangement of GspC and GspD Protomers within the Type 2 Secretion System
Wang X Gu S Guschinskaya N Pickersgill RW
Journal of Biological Chemistry vol. 287 (23), 19082-19093.  

DOI 10.1074/jbc.M112.346338

01-01-2012

An enzyme-trap approach allows isolation of intermediates in cobalamin biosynthesis
Deery E Schroeder S Lawrence AD Taylor SL Seyedarabi A Brown D Geeves MA Howard MJ
Nature Chemical Biology vol. 8 (11), 933-940.  

DOI 10.1038/NCHEMBIO.1086

01-01-2012

2011

Joint neutron/X-ray crystallographic study on the mechanism of pectate lyase
Ali S Texeira S
Acta Crystallographica Section A: Foundations and advances. vol. 67 (a1), c734-c734.  

DOI 10.1107/s0108767311081487

22-08-2011

A Novel Phenotype of Glycogen Storage Disease Presenting as Gastrointestinal Neuromuscular Disease
Martin JE Chowdhury AH McElwaine S Fikree A Broad J Knowles CH Allen L Bevan S et al.
Gastroenterology, Elsevier vol. 140 (5), s-705.  

DOI 10.1016/s0016-5085(11)62930-2

01-05-2011

Structure of PduT, a trimeric bacterial microcompartment protein with a 4Fe-4S cluster-binding site.
Pang A Warren MJ
Acta Crystallogr D Biol Crystallogr vol. 67 (Pt 2), 91-96.  

DOI 10.1107/S0907444910050201

01-02-2011

Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization
Romao CV Ladakis D Lobo SAL Carrondo MA Brindley AA Deery E
P Natl Acad Sci Usa vol. 108 (1), 97-102.  

DOI 10.1073/pnas.1014298108

04-01-2011

A Novel Phenotype of Glycogen Storage Disease Presenting as Gastrointestinal Neuromuscular Disease
Martin JE Chowdhury AH McElwaine S Fikree A Broad J Knowles CH Allen L Bevan S et al.
GASTROENTEROLOGY. vol. 140 (5), S705-S705.  
01-01-2011

2010

Cloning, purification and preliminary crystallographic analysis of cobalamin methyltransferases from Rhodobacter capsulatus.
Seyedarabi A Hutchison T To TT Deery E Brindley A
Acta Crystallogr Sect F Struct Biol Cryst Commun vol. 66 (Pt 12), 1652-1656.  

DOI 10.1107/S1744309110042910

01-12-2010

A disulfide driven domain swap switches off the activity of Shigella IpaH9.8 E3 ligase.
Seyedarabi A Sullivan JA Pickersgill RW
Febs Lett vol. 584 (19), 4163-4168.  

DOI 10.1016/j.febslet.2010.09.006

08-10-2010

Millimeter wave spectroscopy of glucose and a calcium-binding protein using a vector network analyser
Shala K Yang B TT T Pickersgill RW Krauss N
35th IRMMW-THZ Internation Conference, 2010 Rome, Italy 5 Sep 2010 - 10 Sep 2010., 1-2.  

DOI 10.1109/ICIMW.2010.5612418

01-10-2010

A 20-residue peptide of the inner membrane protein OutC mediates interaction with two distinct sites of the outer membrane secretin OutD and is essential for the functional type II secretion system in Erwinia chrysanthemi
Login FH Fries M Wang XH Pickersgill RW
Molecular Microbiology, Blackwell Publishing Ltd vol. 76 (4), 944-955.  

DOI 10.1111/j.1365-2958.2010.07149.x

21-04-2010

Structural insights into substrate specificity and the anti beta-elimination mechanism of pectate lyase.
Seyedarabi A To TT Ali S Fries M Madsen R Clausen MH Brocklehurst K
Biochemistry vol. 49 (3), 539-546.  

DOI 10.1021/bi901503g

26-01-2010

2009

Structure of a trimeric bacterial microcompartment shell protein, EtuB, associated with ethanol utilization in Clostridium kluyveri
Heldt D Frank S Seyedarabi A Ladikis D Warren MJ
Biochem J vol. 423, 199-207.  

DOI 10.1042/BJ20090780

15-10-2009

Substrate Recognition
Brocklehurst K Gul S
In Amino Acids, Peptides and Proteins in Organic Chemistry, Wiley 473-504.  

DOI 10.1002/9783527631780.ch11

23-09-2009

Substrate Recognition
Brocklehurst K Gul S Pickersgill RW
In Amino Acids Peptides and Proteins in Organic Chemistry Volume 2 Modified Amino Acids Organocatalysis and Enzymes 473-504.  

DOI 10.1002/9783527631780.ch11

01-01-2009

2008

Enzyme sequence and its relationship to hyperbaric stability of artificial and natural fish lactate dehydrogenases.
Brindley AA Pickersgill RW Partridge JC Dunstan DJ Hunt DM Warren MJ
Plos One vol. 3 (4) 

DOI 10.1371/journal.pone.0002042

QMRO

30-04-2008

Identification, characterization, and structure/function analysis of a corrin reductase involved in adenosylcobalamin biosynthesis
Lawrence AD Deery E McLean KJ Munro AW Rigby SEJ
J Biol Chem vol. 283 (16), 10813-10821.  

DOI 10.1074/jbc.M710431200

18-04-2008

2007

Molecular basis of the activity of the phytopathogen pectin methylesterase.
Fries M Ihrig J Brocklehurst K Pickersgill RW
Embo J vol. 26 (17), 3879-3887.  

DOI 10.1038/sj.emboj.7601816

05-09-2007

Elucidation of substrate specificity in the cobalamin (vitamin B12) biosynthetic methyltransferases. Structure and function of the C20 methyltransferase (CbiL) from Methanothermobacter thermautotrophicus.
Frank S Deery E Brindley AA Leech HK Lawrence A Heathcote P Brocklehurst K
J Biol Chem vol. 282 (33), 23957-23969.  

DOI 10.1074/jbc.M703827200

17-08-2007

2006

Structure of internalin C from Listeria monocytogenes.
Ooi A Hussain S Seyedarabi A
Acta Crystallogr D Biol Crystallogr vol. 62 (Pt 11), 1287-1293.  

DOI 10.1107/S0907444906026746

01-11-2006

Structural and spectroscopic studies shed light on the mechanism of oxalate oxidase.
Opaleye O Rose R-S Whittaker MM Woo E-J Whittaker JW Pickersgill RW
J Biol Chem vol. 281 (10), 6428-6433.  

DOI 10.1074/jbc.M510256200

QMRO

10-03-2006

Characterization of retinaldehyde dehydrogenase 3.
Graham CE Pickersgill RW Warren MJ
Biochem J vol. 394 (Pt 1), 67-75.  

DOI 10.1042/BJ20050918

15-02-2006

2005

Anaerobic synthesis of vitamin B12: characterization of the early steps in the pathway.
Frank S Brindley AA Deery E Heathcote P Lawrence AD Pickersgill RW
Biochem Soc Trans vol. 33 (Pt 4), 811-814.  

DOI 10.1042/BST0330811

01-08-2005

Shigella effector IpaH9.8 binds to a splicing factor U2AF(35) to modulate host immune responses
Okuda J Toyotome T Kataoka N Ohno M Shimura Y Pickersgill R
Biochem Bioph Res Co vol. 333 (2), 531-539.  

DOI 10.1016/j.bbrc.2005.05.145

29-07-2005

2004

The crystal structure of pectate lyase Pel9A from Erwinia chrysanthemi
Jenkins J Hugouvieux-Cotte-Pattat N Pickersgill RW
J Biol Chem vol. 279 (10), 9139-9145.  

DOI 10.1074/jbc.M311390200

05-03-2004

2003

A primordial structure underlying amyloid
Pickersgill RW
Structure vol. 11 (2), 137-138.  

DOI 10.1016/s0969-2126(03)00009-1

01-02-2003

The structures and active sites of pectinases.
PICKERSGILL RW Voragen AGJ Voragen F Schols H Visser R
International symposium on pectins and pectinases (2nd) Rotterdam, The Netherlands 1 Jan 1970., 267-278.  
01-01-2003

2002

Complex cell signaling molecules from ancient molecular glue
Pickersgill RW
Structure vol. 10 (10), 1287-1288.  

DOI 10.1016/s0969-2126(02)00832-8

01-10-2002

Crystal structure of auxin-binding protein 1 in complex with auxin
Woo EJ Marshall J Bauly J Venis M Napier RM
Embo J vol. 21 (12), 2877-2885.  

DOI 10.1093/emboj/cdf291

17-06-2002

A putative three-dimensional targeting motif of polygalacturonase (PehA), a protein secreted through the type II (GSP) pathway in Erwinia carotovora.
Palomäki T Pickersgill R Riekki R
Mol Microbiol vol. 43 (3), 585-596.  

DOI 10.1046/j.1365-2958.2002.02793.x

01-02-2002

2001

Substrate specificity and subsite mobility in T. aurantiacus xylanase 10A.
Lo Leggio L Kalogiannis S Eckert K Bhat MK Andrei C Pickersgill RW
Febs Lett vol. 509 (2), 303-308.  

DOI 10.1016/s0014-5793(01)03177-5

07-12-2001

The architecture of parallel beta-helices and related folds
Jenkins J
Prog Biophys Mol Bio vol. 77 (2), 111-175.  

DOI 10.1016/s0079-6107(01)00013-x

01-10-2001

Crystal structure of mannanase 26A from Pseudomonas cellulosa and analysis of residues involved in substrate binding.
Hogg D Woo EJ Bolam DN McKie VA Pickersgill RW
J Biol Chem vol. 276 (33), 31186-31192.  

DOI 10.1074/jbc.M010290200

17-08-2001

Anisotropic refinement of the structure of Thermoascus aurantiacus xylanase I
Teixeira S Lo Leggio L
Acta Crystallogr D vol. 57, 385-392.  

DOI 10.1107/s0907444900019089

01-03-2001

Three-dimensional structure of Erwinia chrysanthemi pectin methylesterase reveals a novel esterase active site.
Jenkins J Mayans O Pickersgill RW
J Mol Biol vol. 305 (4), 951-960.  

DOI 10.1006/jmbi.2000.4324

26-01-2001

2000

Crystallization and preliminary X-ray analysis of the auxin receptor ABP1
Woo E-J Bauly J Chen J-G Marshall J Macdonald H Goodenough P Pickersgill R
Acta Crystallographica Section D, Structural Biology, International Union of Crystallography (Iucr) vol. 56 (11), 1476-1478.  

DOI 10.1107/s0907444900010714

01-11-2000

Germin is a manganese containing homohexamer with oxalate oxidase and superoxide dismutase activities
Woo E-J Dunwell JM Goodenough PW Marvier AC
Nature Structural & Molecular Biology, Springer Nature vol. 7 (11), 1036-1040.  

DOI 10.1038/80954

01-11-2000

X‐ray crystallographic study of xylopentaose binding to Pseudomonas fluorescens xylanase A
Leggio LL Jenkins J Pickersgill RW
Proteins Structure Function and Bioinformatics, Wiley vol. 41 (3), 362-373.  

DOI 10.1002/1097-0134(20001115)41:3<362::aid-prot80>3.0.co;2-n

01-01-2000

1999

Xylanase–oligosaccharide interactions studied by a competitive enzyme assay
Leggio LL
Enzyme and Microbial Technology, Elsevier vol. 25 (8-9), 701-709.  

DOI 10.1016/s0141-0229(99)00103-9

01-11-1999

High resolution structure and sequence of T. aurantiacus Xylanase I: Implications for the evolution of thermostability in family 10 xylanases and enzymes with βα‐barrel architecture
Leggio LL Bhat MK
Proteins Structure Function and Bioinformatics, Wiley vol. 36 (3), 295-306.  

DOI 10.1002/(sici)1097-0134(19990815)36:3<295::aid-prot4>3.0.co;2-6

15-08-1999

Ferulic acid esterase‐III from Aspergillus niger does not exhibit lipase activity
Aliwan FO Kroon PA Faulds CB Williamson G
Journal of The Science of Food and Agriculture, Wiley vol. 79 (3), 457-459.  

DOI 10.1002/(sici)1097-0010(19990301)79:3<457::aid-jsfa283>3.0.co;2-g

01-03-1999

Crystallization and preliminary X-ray analysis of arabinanase A from Pseudomonas fluorescens subspecies cellulosa
Scott M Pickersgill RW Hazlewood GP Gilbert HJ
Acta Crystallographica Section D, Structural Biology, International Union of Crystallography (Iucr) vol. 55 (Pt 2), 544-546.  

DOI 10.1107/s0907444998011500

01-02-1999

Crystallization and preliminary crystallographic analysis of the endo-polygalacturonase from Erwinia carotovora ssp. carotovora
Pickersgill R Smith D Worboys K Jenkins J
Acta Crystallographica Section D, Structural Biology, International Union of Crystallography (Iucr) vol. 55 (Pt 1), 320-322.  

DOI 10.1107/s0907444998010816

01-01-1999

1998

Barley oxalate oxidase is a hexameric protein related to seed storage proteins: evidence from X‐ray crystallography
Woo E-J Dunwell JM Goodenough PW
Febs Letters, Wiley vol. 437 (1-2), 87-90.  

DOI 10.1016/s0014-5793(98)01203-4

16-10-1998

Crystal Structure of Polygalacturonase from Erwinia carotovora ssp. carotovora *
Pickersgill R Smith D
Journal of Biological Chemistry, Elsevier vol. 273 (38), 24660-24664.  

DOI 10.1074/jbc.273.38.24660

01-09-1998

Superfamilies: the 4/7 superfamily of βα-barrel glycosidases and the right-handed parallel β-helix superfamily
Pickersgill R Harris G Leggio LL Mayans O
Biochemical Society Transactions. vol. 26 (2), 190-197.  

DOI 10.1042/bst0260190

01-05-1998

Chemical and thermal stability of ferulic acid (feruloyl) esterases from Aspergillus
Faulds CB Aliwan FO de Vries RP Pickersgill RW Visser J
Progress in Biotechnology, Elsevier vol. 15, 41-46.  

DOI 10.1016/s0921-0423(98)80007-5

01-01-1998

Crystallization and preliminary X-ray diffraction studies of a family 26 endo-[beta]-1,4 mannanase (ManA) from Pseudomonas fluorescens subspecies cellulosa
Scott M Pickersgill RW Hazlewood GP Gilbert HJ
Acta Crystallographica Section D, Structural Biology, International Union of Crystallography (Iucr) vol. 54 (Pt 1), 129-131.  

DOI 10.1107/s0907444997009062

01-01-1998

Structure and Evolution of Parallel β-Helix Proteins
Jenkins J Pickersgill R
Journal of Structural Biology, Elsevier vol. 122 (1-2), 236-246.  

DOI 10.1006/jsbi.1998.3985

01-01-1998

1997

Structural basis of the properties of an industrially relevant thermophilic xylanase
Harris GW Pickersgill RW Connerton I Touzel J Breton C
Proteins Structure Function and Bioinformatics, Wiley vol. 29 (1), 77-86.  

DOI 10.1002/(sici)1097-0134(199709)29:1<77::aid-prot6>3.0.co;2-c

01-09-1997

Crystallization and preliminary X‐ray analysis of the major endoglucanase from Thermoascus aurantiacus
Lo Leggio L Parry NJ Claeyssens M Bhat MK
Acta Crystallographica Section D, Structural Biology, International Union of Crystallography (Iucr) vol. 53 (5), 599-604.  

DOI 10.1107/s0907444997005404

01-09-1997

Calcium Protects a Mesophilic Xylanase from Proteinase Inactivation and Thermal Unfolding*
Spurway TD Morland C Cooper A Sumner I O'Donnell AG Pickersgill RW
Journal of Biological Chemistry, Elsevier vol. 272 (28), 17523-17530.  

DOI 10.1074/jbc.272.28.17523

01-07-1997

Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases
Mayans O Scott M Connerton I Gravesen T Benen J
Structure, Elsevier vol. 5 (5), 677-689.  

DOI 10.1016/s0969-2126(97)00222-0

01-05-1997

Key Residues in Subsite F Play a Critical Role in the Activity of Pseudomonas fluorescens Subspecies cellulosa Xylanase A Against Xylooligosaccharides but Not Against Highly Polymeric Substrates such as Xylan*
Charnock SJ Lakey JH Virden R Hughes N Sinnott ML Hazlewood GP
Journal of Biological Chemistry, Elsevier vol. 272 (5), 2942-2951.  

DOI 10.1074/jbc.272.5.2942

01-01-1997

1996

The prosequence of procaricain forms an α-helical domain that prevents access to the substrate-binding cleft
Groves MR Taylor MA Scott M Cummings NJ
Structure, Elsevier vol. 4 (10), 1193-1203.  

DOI 10.1016/s0969-2126(96)00127-x

01-10-1996

Crystal structure of a caricain D158E mutant in complex with E‐64
Katerelos NA Taylor MAJ Scott M Goodenough PW
Febs Letters, Wiley vol. 392 (1), 35-39.  

DOI 10.1016/0014-5793(96)00697-7

19-08-1996

Crystallization and preliminary X-ray analysis of pectin lyase A from Aspergillus niger
Jenkins J Scott M Mayans O Harris G Connerton I
Acta Crystallographica Section D, Structural Biology, International Union of Crystallography (Iucr) vol. 52 (2), 402-404.  

DOI 10.1107/s0907444995012698

01-03-1996

Refined Crystal Structure of the Catalytic Domain of Xylanase A from Pseudomonas fluorescens at 1.8 Å Resolution
Harris GW Jenkins JA Connerton I
Acta Crystallographica Section D, Structural Biology, International Union of Crystallography (Iucr) vol. 52 (2), 393-401.  

DOI 10.1107/s0907444995013540

01-03-1996

1995

β‐Glucosidase, β‐galactosidase, family A cellulases, family F xylanases and two barley glycanases form a superfamily of enzymes wit 8‐fold β/α architecture and with two conserved glutamates near the carboxy‐terminal ends of β‐strands four and seven
Jenkins J Leggio LL Harris G
Febs Letters, Wiley vol. 362 (3), 281-285.  

DOI 10.1016/0014-5793(95)00252-5

10-04-1995

The potent mitogen Pasteurella multocida toxin is highly resistant to proteolysis but becomes susceptible at lysosomal pH
Smyth MG Lax AJ
Febs Letters, Wiley vol. 360 (1), 62-66.  

DOI 10.1016/0014-5793(95)00077-m

20-02-1995

1994

Structure of the catalytic core of the family F xylanase from Pseudomonas fluorescens and identification of the xylopentaose-binding sites
Harris GW Jenkins JA Connerton I Cummings N Leggio LL Scott M Gilbert HJ
Structure, Elsevier vol. 2 (11), 1107-1116.  

DOI 10.1016/s0969-2126(94)00112-x

01-11-1994

The structure of Bacillus subtilis pectate lyase in complex with calcium
Pickersgill R Jenkins J Harris G
Nature Structural & Molecular Biology, Springer Nature vol. 1 (10), 717-723.  

DOI 10.1038/nsb1094-717

01-10-1994

Making a small enzyme smaller; removing the conserved loop structure of hen lysozyme
Pickersgill R Varvill K Jones S Fischer B Henderson I Garrard S
Febs Letters, Wiley vol. 347 (2-3), 199-202.  

DOI 10.1016/0014-5793(94)00543-5

27-06-1994

An unequivocal example of cysteine proteinase activity affected by multiple electrostatic interactions
Taylor MAJ Baker KC Connerton IF Cummings NJ Harris GW Henderson IMJ Jones ST
Protein Engineering Design and Selection, Oxford University Press (OUP) vol. 7 (10), 1267-1276.  

DOI 10.1093/protein/7.10.1267

01-01-1994

1993

Modification of the head-group selectivity of porcine pancreatic phospholipase A2 by protein engineering.
Bhat MK Pickersgill RW Perry BN Brown RA Jones ST Mueller-Harvey I
Biochemistry, American Chemical Society (Acs) vol. 32 (45), 12203-12208.  

DOI 10.1021/bi00096a033

01-11-1993

Crystal structure determination and refinement at 2.3-A resolution of the lentil lectin.
Loris R Steyaert J Maes D Lisgarten J
Biochemistry, American Chemical Society (Acs) vol. 32 (34), 8772-8781.  

DOI 10.1021/bi00085a007

31-08-1993

Kinetic analysis of papaya proteinase Ω
Sumner IG Vaughan A Eisenthal R Pickersgill RW Owen AJ
Biochimica Et Biophysica Acta, Elsevier vol. 1164 (3), 243-251.  

DOI 10.1016/0167-4838(93)90255-p

01-08-1993

Factors effecting the thermostability of cysteine proteinases from Carica papaya
SUMNER IG TAYLOR MAJ PICKERSGILL RW
The Febs Journal, Wiley vol. 214 (1), 129-134.  

DOI 10.1111/j.1432-1033.1993.tb17904.x

01-05-1993

Nucleotide sequence and expression in Eschericia coli of cDNAs encoding papaya proteinase omega from Carica papaya
Reveil DF Cummings NJ Baker KC Collins ME Taylor MAJ Sumner IG Pickersgill RW
Gene, Elsevier vol. 127 (2), 221-225.  

DOI 10.1016/0378-1119(93)90723-g

01-05-1993

Crystals of β-Xylanase from Aspergillus oryzae
Golubev AM Neustroev KN
Journal of Molecular Biology, Elsevier vol. 230 (2), 661-663.  

DOI 10.1006/jmbi.1993.1177

01-03-1993

Crystallization and Preliminary X-ray Analysis of a Thermophilic Bacillus Xylanase
Pickersgill RW Debeire P
Journal of Molecular Biology, Elsevier vol. 230 (2), 664-666.  

DOI 10.1006/jmbi.1993.1178

01-03-1993

Molecular dynamics simulation of a phospholipase A2-substrate complex
Jones ST Ahlström P Berendsen HJC
Biochimica Et Biophysica Acta, Elsevier vol. 1162 (1-2), 135-142.  

DOI 10.1016/0167-4838(93)90139-i

01-03-1993

Crystallization and Preliminary X-ray Analysis of the Catalytic Domain of Xylanase A from Pseudomonas fluorescens subspecies cellulosa
Pickersgill RW Jenkins JA Connerton I
Journal of Molecular Biology, Elsevier vol. 229 (1), 246-248.  

DOI 10.1006/jmbi.1993.1023

01-01-1993

Design and engineering of hydrolytic enzymes for enhanced performance in the agricultural and food industries
Pickersgill RW
Journal of Chemical Technology & Biotechnology, Wiley vol. 57 (3), 290-291.  

DOI 10.1002/jctb.280570320

01-01-1993

Corrections: Crystal Structure Determination and Refinement at 2.3-Å Resolution of the Lentil Lectin (Biochemistry (1993) 32(34) (8772–8781) (10.1021/bi00085a007))
Loris R Steyaert J Maes D Lisgarten J
Biochemistry vol. 32 (51) 

DOI 10.1021/bi00214a024

01-01-1993

1992

Crystallization and preliminary X-ray studies of the pectate lyase from Bacillus subtilis
Jenkins J Nasser W Scott M Pickersgill R Vignon J-C
Journal of Molecular Biology, Elsevier vol. 228 (4), 1255-1258.  

DOI 10.1016/0022-2836(92)90330-m

01-12-1992

Structure of monoclinic papain at 1.60 Å resolution
Pickersgill RW Harris GW
Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials, International Union of Crystallography (Iucr) vol. 48 (1), 59-67.  

DOI 10.1107/s0108768191006572

01-02-1992

The segmented anisotropic refinement of monoclinic papain by the application of the rigid‐body TLS model and comparison to bovine ribonuclease A
Harris GW Howlin B Moss DS
Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials, International Union of Crystallography (Iucr) vol. 48 (1), 67-75.  

DOI 10.1107/s0108768191006663

01-02-1992

Two crystal forms of the lentil lectin diffract to high resolution
Loris R Lisgarten J Maes D Körber F
Journal of Molecular Biology, Elsevier vol. 223 (2), 579-581.  

DOI 10.1016/0022-2836(92)90672-7

01-01-1992

1991

Determination of the structure of papaya protease omega
Pickersgill RW Rizkallah P Harris GW
Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials, International Union of Crystallography (Iucr) vol. 47 (5), 766-771.  

DOI 10.1107/s0108768191003191

01-10-1991

Modification of the stability of phospholipase A2 by charge engineering
Pickersgill RW Sumner IG Collins ME Warwicker J Bhat KM
Febs Letters, Wiley vol. 281 (1-2), 219-222.  

DOI 10.1016/0014-5793(91)80397-l

09-04-1991

A novel method for the purification of porcine phospholipase A2 expressed in E.coli
Bhat KM Sumner IG Collins ME Pickersgill RW
Biochemical and Biophysical Research Communications, Elsevier vol. 176 (1), 371-377.  

DOI 10.1016/0006-291x(91)90934-y

01-04-1991

Changes in activity of porcine phospholipase A2 brought about by charge engineering of a major structural element to alter stability
Goodenough PW Bhat KM Perry BN Pickersgill RW Sumner IG Verheij HM
Protein Engineering Design and Selection, Oxford University Press (OUP) vol. 4 (8), 929-934.  

DOI 10.1093/protein/4.8.929

01-01-1991

Enzyme engineering
Pickersgill RW Goodenough PW
Trends in Food Science & Technology, Elsevier vol. 2, 122-126.  

DOI 10.1016/0924-2244(91)90647-2

01-01-1991

1990

Preliminary crystallographic data for protease Ω
PICKERSGILL RW SUMNER IG
The Febs Journal, Wiley vol. 190 (2), 443-444.  

DOI 10.1111/j.1432-1033.1990.tb15594.x

01-06-1990

1989

Structural and electrostatic differences between actinidin and papain account for differences in activity
Pickersgill RW Sumner IG Goodenough PW
Biochemical Journal, Portland Press vol. 257 (1), 310-312.  

DOI 10.1042/bj2570310

01-01-1989

1988

The electrostatic fields in the active-site clefts of actinidin and papain
Pickersgill RW Goodenough PW Sumner IG
Biochemical Journal, Portland Press vol. 254 (1), 235-238.  

DOI 10.1042/bj2540235

15-08-1988

A rapid method of calculating charge–charge interaction energies in proteins
Pickersgill RW
Protein Engineering Design and Selection, Oxford University Press (OUP) vol. 2 (3), 247-248.  

DOI 10.1093/protein/2.3.247

01-01-1988

1987

One‐dimensional disorder in spinach ribulose bisphosphate carboxylase crystals
Pickersgill RW
Acta Crystallographica Section a: Foundations and Advances, International Union of Crystallography (Iucr) vol. 43 (4), 502-506.  

DOI 10.1107/s0108767387099112

01-07-1987

1983

Structure-function relationships of NAD+- and NADP+-dependent dehydrogenases with particular reference to the three-dimensional structure of 6-phosphogluconate dehydrogenase
ADAMS MJ GOVER S PICKERSGILL RW
Biochemical Society Transactions, Portland Press vol. 11 (4), 429-435.  

DOI 10.1042/bst0110429

01-08-1983

The three dimensional structure of sheep liver 6‐phosphogluconate dehydrogenase at 2.6 A resolution.
Adams MJ Archibald IG Carne A Gover S Helliwell JR
The Embo Journal, Springer Nature vol. 2 (6), 1009-1014.  

DOI 10.1002/j.1460-2075.1983.tb01535.x

01-06-1983

Extracellular Vesicle-Linked Vitamin B12 Acquisition via Novel Binding Proteins in Bacteroides thetaiotaomicron
Juodeikis R Ulrich R Clarke C Banasik M Deery E Saalbach G Kräutler B Carding SR et al.
In Biorxiv 

DOI 10.1101/2025.07.31.667871

Scaffolding protein GspB/OutB facilitates assembly of the Dickeya dadantii type 2 secretion system by anchoring the outer membrane secretin pore to the inner membrane and to the peptidoglycan cell wall
Zhang S Gu S Rycroft P Ruaudel F Delolme F Robert X Ballut L Pickersgill RW et al.
In Biorxiv 

DOI 10.1101/2021.08.06.455404

Enzyme encapsulation peptides bind to the groove between tessellating subunits of the bacterial microcompartment shell
Bradley-Clarke J Gu S Rose R-S Warren MJ Pickersgill RW
In Biorxiv 

DOI 10.1101/2022.12.30.522206

Grants

Grants of specific relevance to the Centre for Molecular Cell Biology

BBSRC AIDD CTP CASE Award: Yu-Yuan Yang
Richard Pickersgill and Arianna Fornili
£8,600 Evotec EVOTEC (UK) LIMITED
01-10-2023 - 30-09-2027

Vitamin scavenging in the gut: Structure/function of the tight-binding B12 foraging machinery in Bacteroides - and its biotechnological applications
Richard Pickersgill
£283,203 BBSRC Biotechnology and Biological Sciences Research Council
01-04-2023 - 31-03-2026

Structure of the assembly platform of the type II secretion system
Richard Pickersgill, Vidya Chandran Darbari and Arianna Fornili
£579,168 Biotechnology and Biological Sciences Research Council
28-10-2022 - 31-03-2026

---

Highthroughput Light Sheet Microscopy: Imaging Across Scales
Viji Draviam Sastry, Caroline Brennan, Vidya Chandran Darbari, Chema Martín-Durán, Ivan Kadurin, Isabel M Palacios, Richard Pickersgill, Sarah McClelland, Tyson Sharp and Martin Knight
£386,205 MRC Medical Research Council
01-10-2022 - 31-03-2023

Super-Resolution Microscopy of live cells in 3D
Viji Draviam Sastry, Conrad Mullineaux, Vidya Chandran Darbari, Lakxmi Subramanian, Fabrice Caudron, Richard Pickersgill, Guy Hanke, Alexander Ruban, Peter Thorpe, Christopher Duffy, Chema Martín-Durán, John Connelly, Peter McCormick, James Chapple, Vardhman Rakyan, Tyson Sharp, Susana Alves Godinho and David Lee
£369,201 BBSRC Biotechnology and Biological Sciences Research Council
01-12-2020 - 31-03-2022

Cryo-electron micrscope
Richard Pickersgill
£302,000 BBSRC Biotechnology and Biological Sciences Research Council
08-08-2017 - 07-07-2018

BBSRC DTP 2016 (LIDO)`
Richard Pickersgill
£104,696 BBSRC Biotechnology and Biological Sciences Research Council
01-10-2016 - 30-09-2020

Bacterial Microcompartments
Richard Pickersgill
£17,100 Isogenica Ltd
01-10-2014 - 30-09-2018

Bacterial Microcompartments
Richard Pickersgill
£102,126 BBSRC Biotechnology and Biological Sciences Research Council
01-10-2014 - 30-09-2018

Development of Supramolecular Assemblies
Richard Pickersgill
£601,234 BBSRC Biotechnology and Biological Sciences Research Council
01-10-2014 - 19-10-2020

Research Group

PhD Students

• Charlea Clarke
  Structural and Biochemical Characterisation of B12-Binding Proteins in Bacteroides Thetaiotaomicron
• Madalyn Heaton-Ward
  Lido Dtp Sept 2025
• Yu-Yuan Yang
  Machine Learning For Structure-Based Drug & Biologic Discovery—Binding Pocket Classification For Structure-Based Drug Discovery

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