Research
cell division, chromosome segregation, kinetochore, cell cycle
Interests
We perform research to understand the regulation of cell division. Cell division is the process that creates new cells to replace aged or damaged tissues in our body, however, uncontrolled cell division is the defining characteristic of cancer. We aim to determine how cell division is regulated to allow us to control this process. The ultimate aim is to be able to regulate cell division as a treatment for cancer and to promote cell division of stem cells for regenerative medicine.
A central hypothesis of our work is that the position of proteins within the cell controls cell division. We place normal proteins in different locations within the cell, next to new partner proteins, and test the effect upon cell division. Some of the resulting changes promote cell division, while others block it. We then use this knowledge to understand both how the normal process of cell division is controlled and how it may be manipulated, for example in cancer therapy.
For more information see our lab website www.thorpelab.org
Publications
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Publications of specific relevance to the Centre for Molecular Cell Biology
2024
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Peripolli S, Meneguello L, Perrod C, Singh T, Patel H, Rahman ST, Kiso K,
Thorpe P, Calvanese V, Bertoli C and de Bruin RAM (2024).
Oncogenic c-Myc induces replication stress by increasing cohesins chromatin occupancy in a CTCF-dependent manner. Nature Communications,
Springer Nature vol. 15 (1)
21-02-20242023
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Mishra PK, Au W-C, Castineira PG, Ali N, Stanton J, Boeckmann L, Takahashi Y, Costanzo M, Boone C, Bloom KS,
Thorpe PH and Basrai MA (2023).
Misregulation of cell cycle-dependent methylation of budding yeast CENP-A contributes to chromosomal instability. Molecular Biology of the Cell,
American Society for Cell Biology (ASCB) vol. 34 (10)
12-07-2023![Relevant Publication](/common/publications/images/fav.png)
Mishra PK, Ali N, Au W, Boeckmann L, Takahashi Y, Castineira P, Bloom K,
Thorpe P and Basrai M (2023).
Constitutive methylation of budding yeast CENP-A contributes to chromosomal instability. 01-01-20232022
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Nitika , Zheng B, Ruan L, Kline JT, Omkar S, Sikora J, Texeira Torres M, Wang Y, Takakuwa JE, Huguet R, Klemm C, Segarra VA, Winters MJ, Pryciak PM,
Thorpe PH, Tatebayashi K, Li R, Fornelli L and Truman AW (2022).
Comprehensive characterization of the Hsp70 interactome reveals novel client proteins and interactions mediated by posttranslational modifications. PLoS Biology,
Public Library of Science vol. 20 (10), e3001839-e3001839.
21-10-2022![Relevant Publication](/common/publications/images/fav.png)
Klemm C, Howell RSM and
Thorpe PH (2022).
ScreenGarden: a shinyR application for fast and easy analysis of plate-based high-throughput screens. BMC Bioinformatics,
Springer Nature vol. 23 (1)
05-02-20222021
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Klemm C, Wood H, Thomas GH, Ólafsson G, Torres MT and
Thorpe PH (2021).
Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking. Microbial Cell,
Shared Science Publishers OG vol. 8 (12)
06-12-2021![Relevant Publication](/common/publications/images/fav.png)
Mishra PK, Wood H, Stanton J, Au W-C, Eisenstatt JR, Boeckmann L, Sclafani RA, Weinreich M, Bloom KS,
Thorpe PH and Basrai MA (2021).
Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast. Molecular Biology of the Cell,
American Society for Cell Biology (ASCB) vol. 32 (21)
25-08-20212020
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Klemm C,
Thorpe PH and Ólafsson G (2020).
Cell-cycle phospho-regulation of the kinetochore. Current Genetics,
Springer Nature vol. 67 (2), 177-193.
22-11-2020![Relevant Publication](/common/publications/images/fav.png)
Howell RSM, Klemm C,
Thorpe PH and Csikász-Nagy A (2020).
Unifying the mechanism of mitotic exit control in a spatiotemporal logical model. PLOS Biology,
Public Library of Science (PLoS) vol. 18 (11)
12-11-2020![Relevant Publication](/common/publications/images/fav.png)
Herrero E, Stinus S, Bellows E, Berry LK, Wood H and
Thorpe PH (2020).
Asymmetric Transcription Factor Partitioning During Yeast Cell Division Requires the FACT Chromatin Remodeler and Cell Cycle Progression. Genetics vol. 216 (3), 701-716.
01-11-2020![Relevant Publication](/common/publications/images/fav.png)
Ólafsson G and
Thorpe PH (2020).
Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA. PLOS Genetics,
Public Library of Science (PLoS) vol. 16 (8)
18-08-2020![Relevant Publication](/common/publications/images/fav.png)
Berry LK, Thomas GH and
Thorpe PH (2020).
CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeast. BMC Genomics,
BioMed Central vol. 21 (1)
10-03-2020![Relevant Publication](/common/publications/images/fav.png)
Garcia E, Guo W, Kumar S, Görlitz F, Sparks H, Alexandrov Y, Munro I, Kelly DJ, Warren S, Chennell G, Sardini A, Carling D,
Thorpe P, Dunsby C and French PMW (2020).
FLIM, FRET and high content analysis. SPIE BiOS.
02-03-20202019
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Howell RSM, Csikász-Nagy A and
Thorpe PH (2019).
Synthetic Physical Interactions with the Yeast Centrosome. G3 (Bethesda) vol. 9 (7), 2183-2194.
09-07-2019![bullet icon](/common/publications/images/bullet24.png)
Howell RSM, Csikász-Nagy A and
Thorpe PH (2019).
Synthetic Physical Interactions with the Yeast Centrosome. G3 (Bethesda) vol. 9 (7), 2183-2194.
01-07-2019![bullet icon](/common/publications/images/bullet24.png)
Mishra PK, Olafsson G, Boeckmann L, Westlake TJ, Jowhar ZM, Dittman LE, Baker RE, D'Amours D,
Thorpe PH and Basrai MA (2019).
Cell cycle dependent association of polo kinase Cdc5 with CENP-A contributes to faithful chromosome segregation in budding yeast. Mol Biol Cell, mbcE18090584-mbcE18090584.
06-02-2019![bullet icon](/common/publications/images/bullet24.png)
Guo W, Kumar S, Görlitz F, Garcia E, Alexandrov Y, Munro I, Kelly DJ, Warren S,
Thorpe P, Dunsby C and French P (2019).
Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells. SLAS Technol vol. 24 (3), 308-320.
10-01-20192017
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Ólafsson G and
Thorpe PH (2017).
Rewiring the Budding Yeast Proteome using Synthetic Physical Interactions. Genome Instability 599-612.
17-10-20172016
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Ledesma-Fernández E,
Thorpe PH and de Bruin RAM (2016).
Bringing Functional Genomics into Focus. Cell Systems,
Elsevier BV vol. 3 (3), 214-216.
01-09-2016![bullet icon](/common/publications/images/bullet24.png)
Olafsson G and
Thorpe PH (2016).
Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation Regions. G3-GENES GENOMES GENETICS vol. 6 (8), 2531-2542.
01-08-2016![bullet icon](/common/publications/images/bullet24.png)
Berry LK, Ólafsson G, Ledesma-Fernández E and
Thorpe PH (2016).
Synthetic protein interactions reveal a functional map of the cell. eLife,
eLife vol. 5
21-04-2016![bullet icon](/common/publications/images/bullet24.png)
Herrero E and
Thorpe PH (2016).
Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2. PLOS Genetics,
Public Library of Science (PLoS) vol. 12 (2)
18-02-20162015
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Ólafsson G and
Thorpe PH (2015).
Synthetic physical interactions map kinetochore regulators and regions sensitive to constitutive Cdc14 localization. Proceedings of the National Academy of Sciences of the United States of America,
Proceedings of the National Academy of Sciences vol. 112 (33), 10413-10418.
03-08-2015![bullet icon](/common/publications/images/bullet24.png)
Ledesma-Fernández E and
Thorpe PH (2015).
Fluorescent foci quantitation for high-throughput analysis. Journal of Biological Methods,
Journal of Biological Methods vol. 2 (2)
19-07-20152012
Thorpe PH and Rothstein R (2012).
Visualizing global effects of the DNA damage response. Nature Cell Biology,
Springer Nature vol. 14 (9), 900-901.
01-09-2012
Thorpe PH, Dittmar JC and Rothstein R (2012).
ScreenTroll: a searchable database to compare genome-wide yeast screens. Database,
Oxford University Press (OUP) vol. 2012
01-01-20122011
Thorpe PH, Alvaro D, Lisby M and Rothstein R (2011).
Bringing Rad52 foci into focus. Journal of Cell Biology,
Rockefeller University Press vol. 194 (5), 665-667.
05-09-20112009
Thorpe PH, Bruno J and Rothstein R (2009).
Kinetochore asymmetry defines a single yeast lineage. Proceedings of the National Academy of Sciences of the United States of America,
Proceedings of the National Academy of Sciences vol. 106 (16), 6673-6678.
21-04-20092008
Thorpe PH, Bruno J and Rothstein R (2008).
Modeling Stem Cell Asymmetry in Yeast. Cold Spring Harbor Symposia on Quantitative Biology,
Cold Spring Harbor Laboratory vol. 73, 81-88.
01-01-20082007
Thorpe PH, González-Barrera S and Rothstein R (2007).
More is not always better: the genetic constraints of polyploidy. Trends in Genetics,
Elsevier vol. 23 (6), 263-266.
05-04-20072006
Thorpe PH, Marrero VA, Savitzky MH, Sunjevaric I, Freeman TC and Rothstein R (2006).
Cells Expressing Murine RAD52 Splice Variants Favor Sister Chromatid Repair. Molecular and Cellular Biology,
Taylor & Francis vol. 26 (10), 3752-3763.
01-05-2006![bullet icon](/common/publications/images/bullet24.png)
Cagney G, Alvaro D, Reid RJ,
Thorpe PH, Rothstein R and Krogan NJ (2006).
Functional genomics of the yeast DNA-damage response. Genome Biology,
Springer Nature vol. 7 (9)
01-01-20062004
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Simpson A, King J,
Thorpe P, McLachlan G and Sallenave J-M (2004).
Towards Gene Therapy for Inflammatory and Infective Pulmonary Diseases. Current Genomics,
Bentham Science Publishers vol. 5 (4), 365-383.
01-05-20042002
Thorpe P, Stevenson BJ and Porteous DJ (2002).
Optimising gene repair strategies in cell culture. Gene Therapy,
Springer Nature vol. 9 (11), 700-702.
22-05-2002
Thorpe PH, Stevenson BJ and Porteous DJ (2002).
Functional correction of episomal mutations withshort DNA fragments and RNA–DNA oligonucleotides. The Journal of Gene Medicine,
Wiley vol. 4 (2), 195-204.
01-03-20021999
Thorpe PH and Porteous DJ (1999).
Rapid Quantitation of Gene Therapy Specific CFTR Expression Using the Amplification Refractory Mutation System. BioTechniques,
Future Science Group vol. 27 (1), 122-127.
01-07-19991997
Thorpe PH, Ternent D and Murray NE (1997).
The specificity of StySKI, a type I restriction enzyme, implies a structure with rotational symmetry. Nucleic Acids Research,
Oxford University Press (OUP) vol. 25 (9), 1694-1700.
01-05-1997![bullet icon](/common/publications/images/bullet24.png)
Dryden DTF, Cooper LP,
Thorpe PH and Byron O (1997).
The in Vitro Assembly of the EcoKI Type I DNA Restriction/Modification Enzyme and Its in Vivo Implications †. Biochemistry,
American Chemical Society (ACS) vol. 36 (5), 1065-1076.
01-02-1997