Prof Martin Knight
Professor of Mechanobiology
Dean for Research (Faculty of Science and Engineering)
School of Engineering and Materials Science
Queen Mary University of London
Queen Mary University of London
Research
Organ-on-a-Chip, Predictive in vitro Models, Mechanobiology, Primary Cilia, Musculoskeletal Bioengineering, Cancer
Interests
A key aspect of my work explores the development of organ-on-a-chip models for understanding human biology in health and disease and predicting the response to therapeutics. I am the Co-Director of the Queen Mary Centre for Predictive in vitro Models (https://www.cpm.qmul.ac.uk/) and one of the directors of the associated EPSRC Centre for Doctoral Training in Next Generation Organ-on-a-chip Technology.As a Professor of 'Mechanobiology', I am particularly interested in the incorporation of physiological and pathological biomechanical stimuli to improve the predictive power of these in vitro models. My research group work on a variety of different musculoskeletal, cardiovascular and epithelial tissues including cartilage, synovium, bone, kidney and artery, as well as diseases including osteoarthritis, cancer, atherosclerosis and inflammation.
I am also interested in the fundamental mechanisms of how living cells and tissues respond to biomechanical stimuli. This led me to examine the role of the cellular structures known as primary cilia. My group conducted pioneering research showing the involvement of cilia in mechanosignalling and inflammation. We are now investigating whether manipulation of cilia can be used as a therapeutic strategy.
Publications
2023
Verbruggen SW, Nolan J, Duffy MP, Pearce OMT, Jacobs CR and Knight MM (2023). A Novel Primary Cilium‐Mediated Mechanism Through which Osteocytes Regulate Metastatic Behavior of Both Breast and Prostate Cancer Cells. Advanced Science, Wiley vol. 11 (2) 15-11-2023
Thompson CL, Hopkins T, Bevan C, Screen HRC, Wright KT and Knight MM (2023). Human vascularised synovium-on-a-chip: a mechanically stimulated, microfluidic model to investigate synovial inflammation and monocyte recruitment. Biomedical Materials, IOP Publishing vol. 18 (6) 10-10-2023
Nolan J, Pearce OMT, Screen HRC, Knight MM and Verbruggen SW (2023). Organ-on-a-Chip and Microfluidic Platforms for Oncology in the UK. Cancers, MDPI vol. 15 (3) 19-01-2023
2022
Meng H, Thompson CL, Coveney CR, Wann AK and Knight MM (2022). Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes. Cartilage Tissue Engineering 157-176. 11-11-2022
Meng H, Fu S, Ferreira MB, Hou Y, Pearce OM, Gavara N and Knight MM (2022). YAP activation inhibits inflammatory signalling and cartilage breakdown associated with reduced primary cilia expression. Osteoarthritis and Cartilage, Elsevier vol. 31 (5), 600-612. 08-11-2022
Hayward M-K, Allen MD, Gomm JJ, Goulding I, Thompson CL, Knight MM, Marshall JF and Jones JL (2022). Mechanostimulation of breast myoepithelial cells induces functional changes associated with DCIS progression to invasion. npj Breast Cancer, Springer Nature vol. 8 (1) 20-09-2022
Badar W, Ali H, Brooker ON, Newham E, Snow T, Terrill NJ, Tozzi G, Fratzl P, Knight MM and Gupta HS (2022). Collagen pre-strain discontinuity at the bone—Cartilage interface. PLOS ONE, Public Library of Science (PLoS) vol. 17 (9) 15-09-2022
Heywood HK, Gardner L, Knight MM and Lee DA (2022). Oscillations of the circadian clock protein, BMAL-1, align to daily cycles of mechanical stimuli: a novel means to integrate biological time within predictive in vitro model systems. In vitro models, Springer Nature, 1-8. 31-08-2022
2021
Inamdar SR, Prévost S, Terrill NJ, Knight MM and Gupta HS (2021). Reversible changes in the 3D collagen fibril architecture during cyclic loading of healthy and degraded cartilage. Acta Biomaterialia, Elsevier vol. 136, 314-326. 24-11-2021
Malacrida B, Delaine-Smith R, Nichols S, Maniati E, Jones RL, Knight MM, Pearce OM and Balkwill FR (2021). Abstract 2642: Using 3-Dimensional in vitro models to unravel the complexity of the metastatic microenvironment in high-grade serous ovarian cancer. 01-07-2021
Verbruggen SW, Thompson CL, Duffy MP, Lunetto S, Nolan J, Pearce OMT, Jacobs CR and Knight MM (2021). Mechanical Stimulation Modulates Osteocyte Regulation of Cancer Cell Phenotype. Cancers, MDPI vol. 13 (12) 10-06-2021
Malacrida B, Nichols S, Maniati E, Jones R, Delanie-Smith R, Roozitalab R, Tyler EJ, Thomas M, Boot G, Mackerodt J, Lockley M, Knight MM, Balkwill FR and Pearce OMT (2021). A human multi-cellular model shows how platelets drive production of diseased extracellular matrix and tissue invasion. iScience, Elsevier vol. 24 (6) 29-05-2021
Delaine-Smith RM, Maniati E, Malacrida B, Nichols S, Roozitalab R, Jones RR, Lecker LSM, Pearce OMT, Knight MM and Balkwill FR (2021). Modelling TGFβR and Hh pathway regulation of prognostic matrisome molecules in ovarian cancer. iScience, Elsevier vol. 24 (6) 29-05-2021
Thompson C, Mcfie M, Chapple J, BEALES P and Knight M (2021). Polycystin-2 is required for chondrocyte mechanotransduction and traffics to the primary cilium in response to mechanical stimulation. International Journal of Molecular Sciences, MDPI AG 21-04-2021
Wu H, Wang Z, Liu S, Meng H, Shelton JC, Thompson CL, Fu S and Knight MM (2021). Sub-toxic levels of cobalt ions impair mechanostranduction via HDAC6-depedent primary cilia shortening. Biochem Biophys Res Commun 15-04-2021
Thompson CL, Fu S, Heywood HK, Knight MM and Thorpe SD (2021). Corrigendum: Mechanical Stimulation: A Crucial Element of Organ-on-Chip Models. Frontiers in Bioengineering and Biotechnology, Frontiers vol. 9 18-02-2021
Delaine-Smith R, Maniati E, Malacrida B, Nichols S, Roozitalab R, Jones R, Lecker L, Pearce O, Knight M and Balkwill F (2021). Modelling TGFβR and Hh pathway regulation of prognostic matrisome molecules in ovarian cancer., Editors: Balkwill F. biorxiv.org 02-02-2021
Fu S, Meng H, Inamdar S, Das B, Gupta H, Wang W, Thompson C and Knight M (2021). Activation of TRPV4 by mechanical, osmotic or pharmaceutical stimulation is anti-inflammatory blocking IL-1β mediated articular cartilage matrix destruction., Editors: Block JA. Osteoarthritis and Cartilage, Elsevier 01-01-2021
Malacrida B, Delaine-Smith R, Nichols S, Maniati E, Jones RL, Knight MM, Pearce OM and Balkwill FR (2021). Using 3-Dimensional in vitro models to unravel the complexity of the metastatic microenvironment in high-grade serous ovarian cancer. 01-01-2021
2020
Thompson CL, Fu S, Heywood HK, Knight MM and Thorpe SD (2020). Mechanical Stimulation: A Crucial Element of Organ-on-Chip Models. Frontiers in Bioengineering and Biotechnology, Frontiers Media SA vol. 8 10-12-2020
Zhang J, Guo Q, Li X, Li C, Wu K, Abrahams I, Yan H, Knight MM, Humphreys CJ and Su L (2020). Solution-Processed Epitaxial Growth of Arbitrary Surface Nanopatterns on Hybrid Perovskite Monocrystalline Thin Films. ACS Nano, American Chemical Society 27-08-2020
Torres-Pérez JV, Naeem H, Thompson CL, Knight MM and Novak P (2020). Nanoscale mapping reveals functional differences in ion channels populating the membrane of primary cilia. Cellular Physiology and Biochemistry vol. 54 (1), 15-26. 10-01-2020
2019
Fu S, Meng H, Freer F, Kwon J, Shelton JC and Knight MM (2019). Sub-toxic levels of Co2+ are anti-inflammatory and protect cartilage from degradation caused by IL-1β. Clinical Biomechanics, Elsevier vol. 79 24-12-2019
Walker RV, Keynton JL, Grimes DT, Sreekumar V, Williams DJ, Esapa C, Wu D, Knight MM and Norris DP (2019). Ciliary exclusion of Polycystin-2 promotes kidney cystogenesis in an autosomal dominant polycystic kidney disease model. Nature Communications, Springer Nature vol. 10 (1) 06-09-2019
Inamdar SR, Barbieri E, Terrill NJ, Knight MM and Gupta HS (2019). Proteoglycan degradation mimics static compression by altering the natural gradients in fibrillar organisation in cartilage. Acta Biomaterialia, Elsevier vol. 97, 437-450. 30-07-2019
Delaine-Smith R, Wright N, Hanley C, Hanwell R, Bhome R, Bullock M, Drifka C, Eliceiri K, Thomas G, Knight M, Mirnezami A and Peake N (2019). Transglutaminase-2 Mediates the Biomechanical Properties of the Colorectal Cancer Tissue Microenvironment that Contribute to Disease Progression. Cancers, MDPI AG 21-05-2019
Fu S, Thompson CL, Ali A, Wang W, Chapple JP, Mitchison HM, Beales PL, Wann AKT and Knight MM (2019). Mechanical loading inhibits cartilage inflammatory signalling via an HDAC6 and IFT-dependent mechanism regulating primary cilia elongation. Osteoarthritis and Cartilage, Elsevier vol. 27 (7), 1064-1074. 25-03-2019
Harris C, THORPE SD, Rushwan S, Wang W, THOMPSON C, Peacock J, KNIGHT M, Gooptu B and Greenough A (2019). An in vitro investigation of the inflammatory response to the strain amplitudes which occur during high frequency oscillation ventilation and conventional mechanical ventilation. Journal of Biomechanics, Elsevier 22-03-2019
Delaine-Smith R, Wright N, Hanley CJ, Hanwell R, Bhome R, Bullock M, Drifka CR, Eliceiri KW, Thomas G, Knight MM, Mirnezami AH and Peake NJ (2019). Transglutaminase-2 mediates stromal biomechanics in colorectal cancer. 01-01-2019
2018
O’Toole SM, Watson DS, Novoselova TV, Romano LEL, King PJ, Bradshaw TY, Thompson CL, Knight MM, Sharp TV, Barnes MR, Srirangalingam U, Drake WM and Chapple JP (2018). Oncometabolite induced primary cilia loss in pheochromocytoma. Endocrine Related Cancer, Bioscientifica vol. 26 (1), 165-180. 05-09-2018
Rowson DT, Shelton JC, Screen HRC and Knight MM (2018). Mechanical loading induces primary cilia disassembly in tendon cells via TGFβ and HDAC6. Scientific Reports, Springer Nature vol. 8 (1) 23-07-2018
Xu J, Nyga A, Li W, Zhang X, Gavara N, Knight MM and Shelton JC (2018). Cobalt ions stimulate a fibrotic response through matrix remodelling, fibroblast contraction and release of pro-fibrotic signals from macrophages. European Cells and Materials vol. 36, 142-155. 01-07-2018
Kenny FN, Drymoussi Z, Delaine-Smith R, Kao AP, Laly AC, Knight MM, Philpott MP and Connelly JT (2018). Tissue stiffening promotes keratinocyte proliferation through activation of epidermal growth factor signaling. Journal of Cell Science vol. 131 (10), 215780-215780. 15-05-2018
Xu J, Yang J, Nyga A, Ehteramyan M, Moraga A, Wu Y, Zeng L, Knight MM and Shelton JC (2018). Cobalt (II) ions and nanoparticles induce macrophage retention by ROS-mediated down-regulation of RhoA expression. Acta Biomater vol. 72, 434-446. 09-04-2018
Pearce OMT, Nichols S, Delaine-Smith R, Jones RR, Maniati E, Rootizalab R, Mackerodt J, Knight MM and Balkwill FR (2018). Investigating drivers of the tumour matrisome, and their role in disease progression. 01-01-2018
2017
Pearce OMT, Delaine-Smith R, Maniati E, Nichols S, Wang J, Böhm S, Rajeeve V, Ullah D, Chakravarty P, Jones RR, Montfort A, Dowe T, Gribben J, Jones JL, Kocher HM, Serody JS, Vincent BG, Connelly J, Brenton JD, Chelala C, et al. (2017). Deconstruction of a metastatic tumor microenvironment reveals a common matrix response in human cancers. Cancer Discov 01-12-2017
Thompson CL, Plant JC, Wann AK, Bishop CL, Novak P, Mitchison HM, Beales PL, Chapple JP and Knight MM (2017). Chondrocyte expansion is associated with loss of primary cilia and disrupted hedgehog signalling. Eur Cell Mater vol. 34, 128-141. 20-09-2017
Inamdar SR, Knight DP, Terrill NJ, Karunaratne A, Cacho-Nerin F, Knight MM and Gupta HS (2017). The Secret Life of Collagen: Temporal Changes in Nanoscale Fibrillar Pre-Strain and Molecular Organization During Physiological Loading of Cartilage. ACS Nano 11-08-2017
Zhang J, Dalbay MT, Luo X, Vrij E, Barbieri D, Moroni L, de Bruijn JD, van Blitterswijk CA, Chapple JP, Knight MM and Yuan H (2017). Topography of calcium phosphate ceramics regulates primary cilia length and TGF receptor recruitment associated with osteogenesis. Acta Biomater vol. 57, 487-497. 15-07-2017
Balkwill FR, Pearce OM, Delaine-Smith R, Maniati E, Nichols S, Wang J, Bessant C and Knight M (2017). Abstract 5558: Deconstruction of a human tumor microenvironment. 01-07-2017
Harris C, Rushwan S, Wang W, Thorpe S, Thompson C, Peacock J, Knight M, Gooptu B and Greenough A (2017). P07 Interleukin response to cyclical mechanical stretch with models of different neonatal ventilation modes. Plenary. 01-05-2017
Gambassi S, Geminiani M, Thorpe SD, Bernardini G, Millucci L, Braconi D, Orlandini M, Thompson CL, Petricci E, Manetti F, Taddei M, Knight MM and Santucci A (2017). Smoothened‐antagonists reverse homogentisic acid‐induced alterations of Hedgehog signaling and primary cilium length in alkaptonuria. Journal of Cellular Physiology, Wiley vol. 232 (11), 3103-3111. 29-03-2017
THORPE SD, Gambassi S, Thompson CL, Chandrakumar C, Santucci A and Knight MM (2017). Reduced Primary Cilia Length and Altered Arl13b Expression Are Associated with Deregulated Chondrocyte Hedgehog Signalling in Alkaptonuria. Journal of Cellular Physiology, John Wiley & Sons Inc. 03-02-2017
Gooptu B, Rushwan S, Wang W, Harris C, Thorpe S, Knight M and Sethi T (2017). A Cd98hc-Dependent Mechanism Mediates Pro-Inflammatory Cytokine Release In Cell And Animal Models Of Lung Injury. 01-01-2017
Thompson CL, Chapple JP, Beales PL, Mitchison HM, Ramachandran M and Knight MM (2017). Polycystins are required for primary cilia-mediated mechanotransduction in chondrocytes. 01-01-2017
Rowson DT, Knight MM and Screen HRC (2017). Cyclic loading leads to primary cilia disassembly in human tenocytes. 01-01-2017
2016
Delaine-Smith RM, Burney S, Balkwill FR and Knight MM (2016). Experimental validation of a flat punch indentation methodology calibrated against unconfined compression tests for determination of soft tissue biomechanics. Journal of the Mechanical Behavior of Biomedical Materials, Elsevier vol. 60, 401-415. 01-07-2016
Sliogeryte K, Botto L, Lee DA and Knight MM (2016). Chondrocyte dedifferentiation increases cell stiffness by strengthening membrane-actin adhesion. Osteoarthritis and Cartilage, Elsevier vol. 24 (5), 912-920. 01-05-2016
Kelly T-AN, Thompson CL, Tan E, Wann AK, Thorpe SD, Chapple JP, Hung CT and Knight MM (2016). Chondrocyte dedifferentiation down regulates mechano-responsiveness and hedgehog signalling associated with changes in primary cilia structure. World Congress of the Osteoarthritis-Research-Society-International (OARSI) on Osteoarthritis 31 Mar 2016 - 3 Apr 2016. 01-04-2016
Thorpe SD, Gambassi S, Patel R, Santucci A and Knight MM (2016). Changes in chondrocyte primary cilia structure and function are associated with altered mechanoregulation in Alkaptonuria. 01-04-2016
Rowson D, Knight MM and Screen HRC (2016). Zonal variation in primary cilia elongation correlates with localized biomechanical degradation in stress deprived tendon. J Orthop Res vol. 34 (12), 2146-2153. 23-03-2016
KNIGHT MM, wang W, wang Z, wann A, thompson C and hassen A (2016). IFT88 influences chondrocyte actin organization and biomechanics. Osteoarthritis and Cartilage 01-03-2016
Thompson CL, Wiles A, Poole CA and Knight MM (2016). Lithium chloride modulates chondrocyte primary cilia and inhibits Hedgehog signaling. FASEB Journal vol. 30 (2), 716-726. 01-02-2016
Sliogeryte K, Thorpe SD, Wang Z, Thompson CL, Gavara N and Knight MM (2016). Differential effects of LifeAct-GFP and actin-GFP on cell mechanics assessed using micropipette aspiration. Journal of Biomechanics, Elsevier vol. 49 (2), 310-317. 25-01-2016
Knight M, Buchholz N and Bourdoumis A (2016). Physiology of urethral flow. Handbook of Urinary Stents: Basic Science and Clinical Applications 219-219. 01-01-2016
2015
Thompson CL, Patel R, Kelly T-AN, Wann AKT, Hung CT, Chapple JP and Knight MM (2015). Hedgehog signalling does not stimulate cartilage catabolism and is inhibited by Interleukin-1β. Arthritis Research & Therapy vol. 17 (1) 24-12-2015
Tan AR, VandenBerg CD, Attur M, Abramson SB, Knight MM, Bulinski JC, Ateshian GA, Cook JL and Hung CT (2015). Cytokine preconditioning of engineered cartilage provides protection against interleukin-1 insult. Arthritis Res Ther vol. 17, 361-361. 14-12-2015
Thompson CL, Poole CA and Knight MM (2015). Lithium chloride modulates chondrocyte primary cilia and inhibits Hedgehog signaling. The FASEB Journal, Federation of American Society of Experimental Biology vol. 100 (4), A39-A40. 23-10-2015
KNIGHT MM, Thompson CL, Yasmin H, Varone A, Wiles A and Poole CA (2015). Lithium chloride prevents interleukin- 1β induced cartilage degradation and loss of mechanical properties. Journal of Orthopaedic Research, Wiley: 12 months 01-10-2015
CHEN J, BADER DL, LEE DA and KNIGHT MM (2015). FINITE ELEMENT ANALYSIS OF MECHANICAL DEFORMATION OF CHONDROCYTE TO 2D SUBSTRATE AND 3D SCAFFOLD. Journal of Mechanics in Medicine and Biology, World Scientific Publishing vol. 15 (05) 01-10-2015
Dalbay MT, Thorpe SD, Connelly JT, Chapple JP and Knight MM (2015). Adipogenic Differentiation of hMSCs is Mediated by Recruitment of IGF‐1r Onto the Primary Cilium Associated With Cilia Elongation. Stem Cells, Oxford University Press (OUP) vol. 33 (6), 1952-1961. 21-05-2015
2014
Sliogeryte K, Thorpe SD, Lee DA, Botto L and Knight MM (2014). Stem cell differentiation increases membrane-actin adhesion regulating cell blebability, migration and mechanics. Scientific Reports, Springer Nature vol. 4 (1) 04-12-2014
Wang Z, Irianto J, Kazun S, Wang W and Knight MM (2014). The rate of hypo-osmotic challenge influences regulatory volume decrease (RVD) and mechanical properties of articular chondrocytes. Osteoarthritis and Cartilage, Elsevier vol. 23 (2), 289-299. 11-11-2014
Rowson D, Knight M and Screen H (2014). 89 Primary Cilia In Tenocytes From The Inter-fascicular Matrix And The Fascicular Matrix. British Journal of Sports Medicine, BMJ vol. 48 (Suppl 2), a58-a59. 01-09-2014
Wann AKT, Chapple JP and Knight MM (2014). The primary cilium influences interleukin-1β-induced NFκB signalling by regulating IKK activity. Cell Signal vol. 26 (8), 1735-1742. 01-08-2014
Irianto J, Lee DA and Knight MM (2014). Quantification of chromatin condensation level by image processing. Med Eng Phys vol. 36 (3), 412-417. 01-03-2014
Thompson CL, Chapple JP and Knight MM (2014). Primary cilia disassembly down-regulates mechanosensitive hedgehog signalling: a feedback mechanism controlling ADAMTS-5 expression in chondrocytes. Osteoarthritis Cartilage vol. 22 (3), 490-498. 01-03-2014
Irianto J, Ramaswamy G, Serra R and Knight MM (2014). Depletion of chondrocyte primary cilia reduces the compressive modulus of articular cartilage. J Biomech vol. 47 (2), 579-582. 22-01-2014
Shaheen T, Edirisinghe T, Gabriel M, Bourdoumis A, Buchholz N and Knight M (2014). In vitro encrustation of a semi-permanent polymer-covered nitinol ureter stent: an artificial urine model. UROLITHIASIS vol. 42 (3), 203-207. 01-01-2014
2013
Hosny NA, Song M, Connelly JT, Ameer-Beg S, Knight MM and Wheeler AP (2013). Correction: Super-Resolution Imaging Strategies for Cell Biologists Using a Spinning Disk Microscope. PLOS ONE, Public Library of Science (PLoS) vol. 8 (12) 30-12-2013
McMurray RJ, Wann AKT, Thompson CL, Connelly JT and Knight MM (2013). Surface topography regulates wnt signaling through control of primary cilia structure in mesenchymal stem cells. Sci Rep vol. 3 18-12-2013
Wann AK, Thompson CL, Chapple JP and Knight MM (2013). Interleukin-1β sequesters hypoxia inducible factor 2α to the primary cilium. Cilia vol. 2 (1) 13-12-2013
Shafat M, Rajakumar K, Syme H, Buchholz N and Knight MM (2013). Stent encrustation in feline and human artificial urine: does the low molecular weight composition account for the difference? Urolithiasis vol. 41 (6), 481-486. 01-11-2013
Irianto J, Swift J, Martins RP, McPhail GD, Knight MM, Discher DE and Lee DA (2013). Osmotic challenge drives rapid and reversible chromatin condensation in chondrocytes. Biophys J vol. 104 (4), 759-769. 19-02-2013
Hosny NA, Song M, Connelly JT, Ameer-Beg S, Knight MM and Wheeler AP (2013). Super-resolution imaging strategies for cell biologists using a spinning disk microscope. PLoS One vol. 8 (10) 01-01-2013
Hernandez-Hernandez V, Pravincumar P, Diaz-Font A, May-Simera H, Jenkins D, Knight M and Beales PL (2013). Bardet-Biedl syndrome proteins control the cilia length through regulation of actin polymerization. HUMAN MOLECULAR GENETICS vol. 22 (19), 3858-3868. 01-01-2013
Hosny NA, Song M, Knight MM and Wheeler AP (2013). Super-resolution imaging strategies for Cell Biologists using a spinning disk microscope. MOLECULAR BIOLOGY OF THE CELL vol. 24 01-01-2013
2012
Prodromou NV, Thompson CL, Osborn DPS, Cogger KF, Ashworth R, Knight MM, Beales PL and Chapple JP (2012). Heat shock induces rapid resorption of primary cilia. Development, The Company of Biologists vol. 139 (24), e2408-e2408. 15-12-2012
Prodromou NV, Thompson CL, Osborn DPS, Cogger KF, Ashworth R, Knight MM, Beales PL and Chapple JP (2012). Heat shock induces rapid resorption of primary cilia. Journal of Cell Science vol. 125 (18), 4297-4305. 01-12-2012
Thompson C, Prodromou N, Osborn D, Ashworth R, Knight M, Beales P and Chapple J (2012). Heat-shock induces rapid resorption of primary cilia. Cilia, Springer Nature vol. 1 (Suppl 1) 01-11-2012
Thompson C, Chapple J and Knight M (2012). Mechanical strain disrupts primary cilia structure and modulates hedgehog signalling in adult chondrocytes. Cilia, Springer Nature vol. 1 (Suppl 1) 01-11-2012
McMurray R and Knight M (2012). Topographical regulation of primary cilia orientation and length in mesenchymal stem cells. Cilia, Springer Nature vol. 1 (Suppl 1) 01-11-2012
Wann A and Knight M (2012). A role for IFT88/the primary cilium in the inflammatory response to interleukin-1. Cilia, Springer Nature vol. 1 (Suppl 1) 01-11-2012
Wann A, Zuo N, Haycraft C, Jensen C, Poole C, McGlashan S and Knight M (2012). The primary cilium conducts chondrocyte mechanotransduction. Cilia, Springer Nature vol. 1 (Suppl 1) 01-11-2012
Hernandez V, Pravincumar P, Diaz-Font A, May-Simera H, Jenkins D, Knight M and Beales P (2012). Bardet-Biedl syndrome proteins control cilia length through regulation of actin polymerisation. Cilia, Springer Nature vol. 1 (Suppl 1) 01-11-2012
Wheeler APS, Morad S, Buchholz N and Knight MM (2012). The Shape of the Urine Stream - From Biophysics to Diagnostics. PLoS ONE vol. 7 (10) 16-10-2012
Chen J, Irianto J, Inamdar S, Pravincumar P, Lee DA, Bader DL and Knight MM (2012). Cell mechanics, structure, and function are regulated by the stiffness of the three-dimensional microenvironment. Biophys J vol. 103 (6), 1188-1197. 19-09-2012
Pravincumar P, Bader DL and Knight MM (2012). Viscoelastic Cell Mechanics and Actin Remodelling Are Dependent on the Rate of Applied Pressure. PLoS ONE vol. 7 (9) 11-09-2012
Wann AKT and Knight MM (2012). Primary cilia elongation in response to interleukin-1 mediates the inflammatory response. Cellular and Molecular Life Sciences vol. 69 (17), 2967-2977. 01-09-2012
Bonzani IC, Campbell JJ, Knight MM, Williams A, Lee DA, Bader DL and Stevens MM (2012). Dynamic compressive strain influences chondrogenic gene expression in human periosteal cells: a case study. J Mech Behav Biomed Mater vol. 11, 72-81. 01-07-2012
Pravincumar P, Bader D and Knight M (2012). PRESSURE RATE SENSITIVE VISCOELASTIC CELL DEFORMATION INVOLVES ACTIN REMODELLING. Journal of Biomechanics, Elsevier vol. 45 01-07-2012
Wann AKT, Zuo N, Haycraft CJ, Jensen CG, Poole CA, McGlashan SR and Knight MM (2012). Primary cilia mediate mechanotransduction through control of ATP-induced Ca2+ signaling in compressed chondrocytes. FASEB Journal vol. 26 (4), 1663-1671. 01-04-2012
Maeda E, Ye S, Wang W, Bader DL, Knight MM and Lee DA (2012). Gap junction permeability between tenocytes within tendon fascicles is suppressed by tensile loading. Biomechanics and Modeling in Mechanobiology vol. 11 (3-4), 439-447. 01-03-2012
Gladman SJ, Huang W, Lim SN, Dyall SC, Boddy S, Kang JX, Knight MM, Priestley JV and Michael-Titus AT (2012). Improved outcome after peripheral nerve injury in mice with increased levels of endogenous Omega-3 polyunsaturated fatty acids. Journal of Neuroscience vol. 32 (2), 563-571. 11-01-2012
Hosny NA, Lee DA and Knight MM (2012). Single photon counting fluorescence lifetime detection of pericellular oxygen concentrations. J Biomed Opt vol. 17 (1) 01-01-2012
Wann AKT, Thompson C and Knight MM (2012). The Role of the Primary Cilium in Chondrocyte Response to Mechanical Loading. Mechanically Gated Channels and their Regulation 405-426. 01-01-2012
McMurray R and Knight MM (2012). Topographical regulation of primary cilia orientation and length in mesenchymal stem cells. Cilia vol. 1 (Suppl 1), P34-P34. 01-01-2012
Thompson CL, Prodromou NV, Osborn DP, Ashworth R, Knight MM, Beales PL and Chapple JP (2012). Heat-shock induces rapid resorption of primary cilia. Cilia vol. 1 (Suppl 1), P52-P52. 01-01-2012
2011
Gladman S, Lim SN, Dyall S, Knight MM, Priestley JV and Michael-Titus AT (2011). Omega-3 fatty acids and acute neurological trauma: A perspective on clinical translation. OCL - Oleagineux Corps Gras Lipides vol. 18 (6), 317-323. 01-11-2011
Gabriel CEL, Nedilko T, Paulose PA, Bach C, Buchholz N and Knight MM (2011). E84 Can Shockwave lithotripsy remove encrustation from ureteric nitinol stents? European Urology Open Science, Elsevier vol. 10 (7) 01-10-2011
Edirisinghe TY, Knight MM, Buchholz N and Bach C (2011). E70 The influence of extracorporeal shock wave lithotripsy on tissue viability around metallic ureteral stents. European Urology Open Science, Elsevier vol. 10 (7) 01-10-2011
Shafat M, Nedilko T, Syme H, Buchholz N and Knight MM (2011). E189 Does the composition of feline urine prevent calcium oxalate encrustation of urethral stents? European Urology Open Science, Elsevier vol. 10 (7), 517-518. 01-10-2011
Nedilko T, Paulose PA, Buchholz N and Knight MM (2011). E75 Comparison of encrustation rates between ureteric metal stents and Double J stents. European Urology Open Science, Elsevier vol. 10 (7) 01-10-2011
Maeda E, Ye S, Wang W, Bader DL, Knight MM and Lee DA (2011). Gap junction permeability between tenocytes within tendon fascicles is suppressed by tensile loading. Biomechanics and Modeling in Mechanobiology, 1-9. 01-01-2011
Lee DA, Knight MM, Campbell JJ and Bader DL (2011). Stem cell mechanobiology. J Cell Biochem vol. 112 (1), 1-9. 01-01-2011
2010
Gladman SJ, Ward RE, Michael-Titus AT, Knight MM and Priestley JV (2010). The effect of mechanical strain or hypoxia on cell death in subpopulations of rat dorsal root ganglion neurons in vitro. Neuroscience vol. 171 (2), 577-587. 01-12-2010
Vaughan NM, Grainger J, Bader DL and Knight MM (2010). The potential of pulsed low intensity ultrasound to stimulate chondrocytes matrix synthesis in agarose and monolayer cultures. Medical and Biological Engineering and Computing vol. 48 (12), 1215-1222. 01-12-2010
Wann AKT, Mistry J, Blain EJ, Michael-Titus AT and Knight MM (2010). Eicosapentaenoic acid and docosahexaenoic acid reduce interleukin-1β-mediated cartilage degradation. Arthritis Research and Therapy vol. 12 (6) 08-11-2010
Heywood HK, Knight MM and Lee DA (2010). Both superficial and deep zone articular chondrocyte subpopulations exhibit the crabtree effect but have different basal oxygen consumption rates. Journal of Cellular Physiology vol. 223 (3), 630-639. 01-06-2010
Garcia M and Knight MM (2010). Cyclic loading opens hemichannels to release ATP as part of a chondrocyte mechanotransduction pathway. Journal of Orthopaedic Research vol. 28 (4), 510-515. 01-04-2010
Ye S, Maeda E, Knight M, Lee D, Bader D and Wang W (2010). A compartment model to evaluate the permeability of gap junctions between tenocytes in tendon fascicles. FASEB JOURNAL vol. 24 01-01-2010
Bader DL and Knight MM (2010). Measuring the biomechanical properties of cartilage cells. 01-01-2010
McGlashan SR, Knight MM, Chowdhury TT, Joshi P, Jensen CG, Kennedy S and Poole CA (2010). Mechanical loading modulates chondrocyte primary cilia incidence and length. Cell Biology International vol. 34 (5), 441-446. 01-01-2010
Archer C, Ralphs J, Säämänen A-M, Arokoski JPA, Jurvelin JS, Kiviranta I, Hinz B, Hunziker EB, Bader DL, Knight MM, Dell’accio F, Vincent TL, Aigner T, Schmitz N, Söder S, McIlwraith CW, Hangody L, Kish G, Koreny T, Hangody LR, et al. (2010). Contributor contact details. Regenerative Medicine and Biomaterials for the Repair of Connective Tissues xiii-xvii. 01-01-2010
Bader DL and Knight MM (2010). 4 Measuring the biomechanical properties of cartilage cells. Regenerative Medicine and Biomaterials for the Repair of Connective Tissues 106-136. 01-01-2010
Maeda E, Ye S, Knight M, Wang W, Bader D and Lee D (2010). 1P338 1J1450 Mechano-regulation of gap junction communications between tenocytes within isolated fascicles(Bioengineering,Oral Presentations,The 48th Annual Meeting of the Biophysical Society of Japan). Seibutsu Butsuri, Biophysical Society of Japan vol. 50 (supplement2) 01-01-2010
2009
Knight MM, Mcglashan SR, Garcia M, Jensen CG and Poole CA (2009). Articular chondrocytes express connexin 43 hemichannels and P2 receptors - A putative mechanoreceptor complex involving the primary cilium? Journal of Anatomy vol. 214 (2), 275-283. 05-02-2009
2008
Bader DL and Knight MM (2008). Biomechanical analysis of structural deformation in living cells. Medical and Biological Engineering and Computing vol. 46 (10), 951-963. 27-08-2008
Pingguan-Murphy B and Knight MM (2008). Mechanosensitive Purinergic Calcium Signalling in Articular Chondrocytes. Mechanosensitive Ion Channels 235-251. 01-01-2008
2007
Campbell JJ, Blain EJ, Chowdhury TT and Knight MM (2007). Loading alters actin dynamics and up-regulates cofilin gene expression in chondrocytes. Biochemical and Biophysical Research Communications vol. 361 (2), 329-334. 21-09-2007
KNIGHT MM, Chowdhury TT, Campbell JJ and Blain EJ (2007). Loading alters actin dynamics and up-regulates cofilin gene expression in chondrocytes. Biochemical and Biophysical Research Communications vol. 361 (21), 329-334. 01-09-2007
Campbell JJ and Knight MM (2007). An improved confocal FRAP technique for the measurement of long-term actin dynamics in individual stress fibers. Microscopy Research and Technique vol. 70 (12), 1034-1040. 01-01-2007
2006
Chowdhury TT and Knight MM (2006). Purinergic pathway suppresses the release of NO and stimulates proteoglycan synthesis in chondrocyte/agarose constructs subjected to dynamic compression. Journal of Cellular Physiology vol. 209 (3), 845-853. 01-12-2006
Pingguan-Murphy B, El-Azzeh M, Bader DL and Knight MM (2006). Cyclic compression of chondrocytes modulates a purinergic calcium signalling pathway in a strain rate- and frequency-dependent manner. Journal of Cellular Physiology vol. 209 (2), 389-397. 01-11-2006
Hakimi O, Knight DP, Knight MM, Grahn MF and Vadgama P (2006). Ultrastructure of insect and spider cocoon silks. Biomacromolecules vol. 7 (10), 2901-2908. 01-10-2006
Bomzon Z, Knight MM, Bader DL and Kimmel E (2006). Mitochondrial dynamics in chondrocytes and their connection to the mechanical properties of the cytoplasm. Journal of Biomechanical Engineering vol. 128 (5), 674-679. 01-10-2006
Ohashi T, Hagiwara M, Bader DL and Knight MM (2006). Intracellular mechanics and mechanotransduction associated with chondrocyte deformation during pipette aspiration. Biorheology vol. 43 (3-4), 201-214. 18-08-2006
Knight MM, Bomzon Z, Kimmel E, Sharma AM, Lee DA and Bader DL (2006). Chondrocyte deformation induces mitochondrial distortion and heterogeneous intracellular strain fields. BIOMECH MODEL MECHAN vol. 5 (2-3), 180-191. 01-06-2006
Knight MM, Toyoda T, Lee DA and Bader DL (2006). Mechanical compression and hydrostatic pressure induce reversible changes in actin cytoskeletal organisation in chondrocytes in agarose. J Biomech vol. 39 (8), 1547-1551. 01-01-2006
Bader DL, Lee DA and Knight MM (2006). Chondrocyte Deformation and Mechanotransduction in Cartilage Model Systems(International Workshop 2). The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME, Japan Society of Mechanical Engineers vol. 2005.18, 2-3. 01-01-2006
Bomzon Z, Knight MM, Bader DL and Kimmel E (2006). Probing mechanical heterogeneity in chondrocytes using passive microtheology. Journal of Biomechanics, Elsevier vol. 39 01-01-2006
2005
Pingguan-Murphy B, Lee DA, Bader DL and Knight MM (2005). Activation of chondrocytes calcium signalling by dynamic compression is independent of number of cycles. Arch Biochem Biophys vol. 444 (1), 45-51. 01-12-2005
2004
Sawae Y, Shelton JC, Bader DL and Knight MM (2004). Confocal analysis of local and cellular strains in chondrocyte-agarose constructs subjected to mechanical shear. Ann Biomed Eng vol. 32 (6), 860-870. 01-06-2004
Lee DA and Knight MM (2004). Mechanical loading of chondrocytes embedded in 3D constructs: in vitro methods for assessment of morphological and metabolic response to compressive strain. Methods Mol Med vol. 100, 307-324. 01-01-2004
Holloway I, Kayser M, Lee DA, Bader DL, Bentley G and Knight MM (2004). Increased presence of cells with multiple elongated processes in osteoarthritic femoral head cartilage. Osteoarthritis Cartilage vol. 12 (1), 17-24. 01-01-2004
Lee DA and Knight MM (2004). Mechanical Loading of Chondrocytes Embedded in 3D Constructs. Cartilage and Osteoarthritis 307-324. 01-01-2004
2003
Knight MM, Roberts SR, Lee DA and Bader DL (2003). Live cell imaging using confocal microscopy induces intracellular calcium transients and cell death. Am J Physiol Cell Physiol vol. 284 (4), C1083-C1089. 01-04-2003
Bader DL, SHELTON JC, Knight MM and Sawae Y (2003). Confocal Analysis of Local and Cellular Strains in Chondrocyte - Agarose Constructs Subjected to Shear. J Biochemical Engineering ASME 01-01-2003
2002
Knight MM, Toyoda T, Bader DL and Lee DA (2002). Mechanical strain and hydrostatic pressure induce reversible changes in actin cytoskeletal organisation in chondrocytes in agarose. 01-12-2002
Knight MM, Roberts SR, Lee DA and Bader DL (2002). Light-induced calcium signalling in chondrocytes. 01-12-2002
Knight MM, van de Breevaart Bravenboer J, Lee DA, van Osch GJVM, Weinans H and Bader DL (2002). Cell and nucleus deformation in compressed chondrocyte-alginate constructs: temporal changes and calculation of cell modulus. Biochim Biophys Acta vol. 1570 (1), 1-8. 15-02-2002
Knight MM, Roberts SR, LEE DA and Bader DL (2002). Light-induced calcium signalling in chondrocytes. TCES 2002 Annual meeting of the UK Tissue and Cell Engineering Society University of Glasgow, Scotland 19 Sep 2002 - 20 Sep 2002. 01-01-2002
Holloway I, Knight MM, LEE DA, Bader DL and Bentley G (2002). Changes in cell morphology and cytoskeletal organisation in osteoarthritic cartilage. ECM III Cartilage & Joint repair Tutorials, Basic Research & Clinical Methods Davos, Switzerland 1 Jul 2002 - 3 Jul 2002. 01-01-2002
Knight MM, Toyoda T, LEE DA and Bader DL (2002). Mechanical strain and hydrostatic pressure induce reversible changes in actin cytoskeletal organisation in chondrocytes in agarose. 48th Annual Meeting of the Orthopaedic Research Society. 01-01-2002
Holloway I, Knight MM, LEE DA, Bader DL and Bentley G (2002). Characterisation of cell morphology, cytoskeletal organization and apoptosis in human osteoarthritic cartilage. 48th Annual Meeting of the Orthopaedic Research Society. 01-01-2002
Bader DL, Ohashi T, Knight MM, Lee DA and Sato M (2002). Deformation properties of articular chondrocytes: a critique of three separate techniques. 01-01-2002
2001
Knight MM, Ross JM, Sherwin AF, Lee DA, Bader DL and Poole CA (2001). Chondrocyte deformation within mechanically and enzymatically extracted chondrons compressed in agarose. Biochim Biophys Acta vol. 1526 (2), 141-146. 03-05-2001
Knight MM, Idowu BD, Lee DA and Bader DL (2001). Temporal changes in cytoskeletal organisation within isolated chondrocytes quantified using a novel image analysis technique. Med Biol Eng Comput vol. 39 (3), 397-404. 01-05-2001
Roberts SR, Knight MM, Lee DA and Bader DL (2001). Mechanical compression influences intracellular Ca2+ signaling in chondrocytes seeded in agarose constructs. J Appl Physiol (1985) vol. 90 (4), 1385-1391. 01-04-2001
Bouten CV, Knight MM, Lee DA and Bade DL (2001). Compressive deformation and damage of muscle cell subpopulations in a model system. Ann Biomed Eng vol. 29 (2), 153-163. 01-02-2001
Knight MM, van de Breevaart Brevenboer J, van Osch G, Weinans H, LEE DA and Bader DL (2001). Temporal changes in cell and nucleus deformation in isolated chondrocytes compressed in alginate scaffolds. 47th Annual Meeting, Orthopaedic Research Society San Francisco, California 25 Feb 2001 - 28 Feb 2001. 01-01-2001
LEE DA, Idowu BD, Knight MM and Bader DL (2001). Mechanical compression influences cytoskeletal oragnisation within chondrocytes seeded in agarose constructs. 47th Annual Meeting, Orthopaedic Research Society San Francisco, California 25 Feb 2001 - 28 Feb 2001. 01-01-2001
2000
Roberts SR, Knight MM, Lee DA and Bader DL (2000). Intracellular - CA2+ signalling in compressed chondrocyte agarose constructs. 01-12-2000
Knight MM, Van De Breevaart Bravenboer J, Bader DL and Lee DA (2000). Compression induced cell and nucleus deformation in chondrocyte-alginate constructs. 01-12-2000
Idowu BD, Knight MM, Bader DL and Lee DA (2000). Confocal analysis of cytoskeletal organisation within isolated chondrocyte sub-populations cultured in agarose. Histochem J vol. 32 (3), 165-174. 01-03-2000
Roberts SR, Knight MM, Lee DA and Bader DL (2000). Intracellular calcium signalling in compressed chondrocyte agarose constructs. 01-02-2000
Knight DP, KNIGHT MM and Vollrath F (2000). Beta transition and stress-induced phase separation in the spinning of spider dragline silk. International Journal of Biological Macromolecules vol. 27 (3), 205-210. 01-01-2000
Lee DA, Knight MM, Bolton JF, Idowu BD, Kayser MV and Bader DL (2000). Chondrocyte deformation within compressed agarose constructs at the cellular and sub-cellular levels. J Biomech vol. 33 (1), 81-95. 01-01-2000
1998
Lee DA, Noguchi T, Knight MM, O'Donnell L, Bentley G and Bader DL (1998). Response of chondrocyte subpopulations cultured within unloaded and loaded agarose. J Orthop Res vol. 16 (6), 726-733. 01-11-1998
Knight MM, Lee DA and Bader DL (1998). The influence of elaborated pericellular matrix on the deformation of isolated articular chondrocytes cultured in agarose. Biochim Biophys Acta vol. 1405 (1-3), 67-77. 21-10-1998
Grants
Epithelial Exchange Surfaces – From organizing principles to novel culture models of the gatekeepers of the body - MSCA-2022-DN Martin KnightMartin Knight, Hazel Screen, Wen Wang, Clare Thompson and Cleo Bishop
£260,676 EPSRC - EU Scheme (01-12-2023 - 30-11-2027)
Re-profiled BRC 2022-27 Final - Precision Musculoskeletal CareJohn Whiteley, Costantino Pitzalis, Martin Knight, Gregory Slabaugh and Xavier Griffin
£3,343,542 NIHR National Institute for Health Research (01-12-2022 - 30-11-2027)
APP13387: Micro-manufacturing of tissue patterned organ-chips for accelerated deployment of new medicinesMartin Knight, Hazel Screen, Julien Gautrot, Timothy Hopkins, John Connelly, Liisa Blowes and Xavier Griffin
£1,787,725 EPSRC Engineering and Physical Sciences Research Council (01-07-2024 - 30-06-2027)
Incorporating physical cues in organ-chip modelsHazel Screen and Martin Knight
£20,000 Emulate Inc. (01-10-2022 - 30-09-2026)
Human synovium-cartilage organ-chip for personalised surgical screening - Tim Hopkins - fellowshipMartin Knight
£311,203 AR-UK Versus Arthritis (01-04-2022 - 30-06-2026)
Development of an Organ-on-a-chip model of polycystic kidney disease using CRISPR modified human kidMartin Knight and Cleo Bishop
£39,986 Kidney Research UK (01-12-2023 - 30-11-2025)
Production of a Human Growth Plate Organ-Chip Model of Skeletal DevelopmentMartin Knight, Clare Thompson and Hazel Screen
£197,923 BBSRC Biotechnology and Biological Sciences Research Council (01-02-2023 - 31-07-2024)
Organ-on-a-chip model of breast cancer bone metastasesMartin Knight and Oliver Pearce
£268,711 CR-UK Cancer Research UK (01-01-2021 - 30-06-2024)
MICA: Organ-on-a-chip models for safety testing of regenerative medicine productsHazel Screen and Martin Knight
£473,325 MRC Medical Research Council (24-08-2020 - 24-05-2024)
Organ-on-a-chip Centre of ExcellenceMartin Knight and Hazel Screen
£420,794 Emulate Inc. (20-08-2019 - 01-01-2024)
Emulate Organs-on-Chips Centre TechnicianMartin Knight, Hazel Screen and Clare Thompson
£49,584 Emulate Inc. (01-01-2021 - 31-12-2023)
Collagen Fibrillar Network in Ageing CartilageHimadri Gupta and Martin Knight
£374,874 BBSRC Biotechnology and Biological Sciences Research Council (01-10-2017 - 24-07-2023)
Highthroughput Light Sheet Microscopy: Imaging Across ScalesViji Draviam Sastry, Caroline Brennan, Vidya Darbari, Jose Martin Duran, Ivan Kadurin, Maria Isabel Palacios De Castro, Richard Pickersgill, Sarah McClelland, Tyson Sharp and Martin Knight
£386,205 MRC Medical Research Council (01-10-2022 - 31-03-2023)
Correlating structural and proteomic techniques on cartilageHimadri Gupta and Martin Knight
£18,515 MRC Medical Research Council (24-07-2022 - 28-02-2023)
Organ-on-a-chip technologiesHazel Screen and Martin Knight
£479,339 MRC Medical Research Council (01-08-2018 - 30-09-2022)