Prof Wen Wang
Professor of Biomedical Engineering
Vice-Principal and Executive Dean for Science and Engineering
School of Engineering and Materials Science
Queen Mary University of London
Queen Mary University of London
Research
Cell biomechanics, Biofluids, Flow and solute transport in tissues, Cardiovascular disease, Arterial haemodynamics, Microcirculation
Interests
My work focuses on vascular bioengineering and biomaterial mechanics, including studies on the endothelial glycocalyx, vascular stem cells and progenitor cells, transmembrane and transcapillary exchange. Working at the interface between biomedical engineering, biomaterials and cell mechanics, I have led a number of multidisciplinary projects in the UK, as well as international projects between the UK, US, China and Japan. Due to limitation of the space, I will only present our research on the vascular endothelial glycocalyx. We have a number of other projects, including microcapsules as vehicles for drug delivery and vascular stem cells in collaboration with colleagues in Medical Schools at Queen Mary and King's College London. Details of these studies can be found in our publication lists.Studies on the glycocalyx
a) its spatial distribution and temporal development on the endothelial cell membrane;
b) mechanical properties of the glycocalyx;
c) effects of fluid shear stress on its distribution on the cell membrane; and
d) its stability and interplay with the cytoskeletal actin network.
The endothelial glycocalyx is a carbohydrate–protein layer that lines the luminal surface of the endothelium. It anchors to the cell membrane via its core proteins that share extended link to the actin cytoskeleton. Those protein domains and the attached carbohydrates are susceptible to pathological changes, contributing to vascular diseases.
a) Spatio-temporal development of the glycocalyx
We observe the spatial distribution of the endothelial glycocalyx layer using laser-scanning confocal microscopy. Cells after different days in culture are studied to evaluate the temporal development of the glycocalyx layer. Comparisons are made between the controls and enzyme-treated groups.
Our in vitro results using confocal microscopy show the development of the endothelial glycocalyx over time. The growth appears to initiate from the edge of cells and takes up to two weeks to cover the apical region of the cell membrane (i.e. region above the nucleus). The mechanism underlining this spatial distribution and temporal change needs further investigation, but it seems functionally beneficial as the glycocalyx layer covers the intercellular gaps for the endothelium to fulfil its role as a selective semi-permeable membrane for the circulating blood. Our three-dimensional reconstructed images, although limited by the confocal resolution in the z-direction, indicate that the thickness of the glycocalyx layer is approximately 1 μm. This finding agrees reasonably well with previous studies.
b) Mechanical property of the glycocalyx
we have investigated mechanical properties of the glycocalyx layer using AFM nano-indentation. The Young's modulus of the glycocalyx is calculated by analysing testing results on the HUVEC cell membrane with and without the glycocalyx layer.
Indentation of the cell membrane of the initial 200 nm (after bending of the cantilever is deducted) enables us to estimate the Young's modulus of the glycocalyx layer by comparing the change in the cell membrane Young's modulus with and without the glycocalyx. We can simplify the flexible glycocalyx layer on a deformable cell membrane to a two springs in series system. Our results suggest that the cell membrane Young's modulus without the glycocalyx layer is approximately 2.13 kPa (average of day 14 and 21), and with the glycocalyx layer, the overall Young's modulus is approximately 0.34 kPa (average of day 14 and 21 control groups). This leads to the Young's modulus of the glycocalyx layer alone to be approx. 0.39 kPa.
c) Shear stress-induced redistribution of the glycocalyx
We study the effects of shear stress on the spatial distribution of the glycocalyx on endothelial cell membranes, using a flow chamber. Two parameters are used to characterize the spatial distribution of the glycocalyx: (a) the percentage area of the cell membrane covered by the glycocalyx layer and (b) the fluorescence intensity ratio between the apical and edge regions of endothelial cells. They give quantitative measures of the observed changes in the distribution of the glycocalyx following shear exposure. Comparisons are made at different time points after shear stimulation to study the redistribution of the glycocalyx on the cell membrane. Further studies are carried out to analyse the recovery of the glycocalyx layer following its enzyme degradation in either static or shear flow conditions.
d) Stability of the glycocalyx
We investigate the contribution of the actin cytoskeleton to the structural stability of the glycocalyx in vitro. Specifically, we establish two states of actin depolymerisation with the use of cytochalasin D (CD). One is rapid depolymerisation which quickly disrupts the actin using a high concentration of CD, and the other is prolonged actin disruption by employing a low CD concentration to persistently depolymerise the actin cytoskeleton.
Publications
2024
Xiao Y, Luo S, Qiu J, Zhang Y, Liu W, Zhao Y, Zhu Y, Deng Y, Lu M, Liu S, Lin Y, Huang A, Wang W, Hu X and Gu B (2024). Highly sensitive SERS platform for pathogen analysis by cyclic DNA nanostructure@AuNP tags and cascade primer exchange reaction. Journal of Nanobiotechnology vol. 22 26-02-2024
2023
Li W, Wang W, Luo S, Chen S, Ji T, Li N, Pan W, Zhang X, Wang X, Li K, Zhang Y and Yan X (2023). A sensitive and rapid electrochemical biosensor for sEV-miRNA detection based on domino-type localized catalytic hairpin assembly. Journal of Nanobiotechnology, Springer Nature vol. 21 (1) 09-09-2023
Chen S, Cheng Y, Zhao Z, Zhang K, Hao T, Sui Y, Wang W, Zhao J and Li Y (2023). Core–Shell-Structured Electrorheological Fluid with a Polarizability-Tunable Nanocarbon Shell for Enhanced Stimuli-Responsive Activity. ACS Applied Materials & Interfaces, American Chemical Society (ACS) vol. 15 (29), 35741-35749. 14-07-2023
Feng J, Jia L, Pan W, Fan Y, Guo J, Luo T, Liu C, Wang W, Zheng L and Li B (2023). Rapid and efficient fluorescent aptasensor for PD-L1 positive extracellular vesicles isolation and analysis: EV-ANCHOR. Chemical Engineering Journal, Elsevier vol. 465 01-06-2023
Guo Z, Lin T, Jing D, Wang W and Sui Y (2023). A method for real-time mechanical characterisation of microcapsules. Biomechanics and Modeling in Mechanobiology, Springer Nature vol. 22 (4), 1209-1220. 24-03-2023
Wang Z, Lu R, Wang W, Tian FB, Feng JJ and Sui Y (2023). A computational model for the transit of a cancer cell through a constricted microchannel. Biomechanics and Modeling in Mechanobiology, Springer Nature vol. 22 (4), 1129-1143. 28-02-2023
Zhang X, Jiang X, Wang W, Luo S, Guan S, Li W, Situ B, Li B, Zhang Y and Zheng L (2023). A simple and sensitive electrochemical biosensor for circulating tumor cell determination based on dual-toehold accelerated catalytic hairpin assembly. Microchimica Acta, Springer Nature vol. 190 (2) 24-01-2023
2022
Zhang Y, Li W, Ji T, Luo S, Qiu J, Situ B, Li B, Zhang X, Zhang T, Wang W, Xiao Y, Zheng L and Yan X (2022). Localized DNA tetrahedrons assisted catalytic hairpin assembly for the rapid and sensitive profiling of small extracellular vesicle-associated microRNAs. Journal of Nanobiotechnology, Springer Nature vol. 20 (1) 01-12-2022
Gao F, Yin J, Long Y, Zhu S, Huang Z, Wang J, Zheng H, Wang W and Zheng L (2022). Diagnostic Value of IGFBP-2 in Predicting Preeclampsia before 20 Weeks of Pregnancy: A Prospective Nested Case-Control Study. Computational and Mathematical Methods in Medicine, Hindawi vol. 2022 28-09-2022
Jia L, Li B, Fang C, Liang X, Xie Y, Sun X, Wang W, Zheng L and Wang D (2022). Extracellular Vesicles of Mesenchymal Stem Cells Are More Effectively Accessed through Polyethylene Glycol-Based Precipitation than by Ultracentrifugation. Stem Cells International, Hindawi vol. 2022 06-09-2022
Wu Y, Yang J, van Teijlingen A, Berardo A, Corridori I, Feng J, Xu J, Titirici MM, Rodriguez-Cabello JC, Pugno NM, Sun J, Wang W, Tuttle T and Mata A (2022). Disinfector-Assisted Low Temperature Reduced Graphene Oxide-Protein Surgical Dressing for the Postoperative Photothermal Treatment of Melanoma. Advanced Functional Materials 08-07-2022
Lin T, Wang Z, Lu RX, Wang W and Sui Y (2022). Characterising Mechanical Properties of Flowing Microcapsules Using a Deep Convolutional Neural Network. Advances in Applied Mathematics and Mechanics, Global Science Press vol. 14 (1), 79-100. 01-06-2022
Zhang Y, Hollis D, Ross R, Snow T, Terrill NJ, Lu Y, Wang W, Connelly J, Tozzi G and Gupta HS (2022). Investigating the Fibrillar Ultrastructure and Mechanics in Keloid Scars Using In Situ Synchrotron X-ray Nanomechanical Imaging. Materials (Basel), MDPI vol. 15 (5) 01-03-2022
2021
Pu JH, Wang SK, Sun J, Wang W and Wang HS (2021). Growth and self-jumping of single condensed droplet on nanostructured surfaces: A molecular dynamics simulation. Journal of Molecular Liquids, Elsevier vol. 340 01-10-2021
Li W and Wang W (2021). The role of actomyosin in the regulation of syndecan-1 in hyperosmosis. BBA - General Subjects, Elsevier vol. 1865 (10) 31-07-2021
Lu RX, Wang Z, Salsac A-V, Barthès-Biesel D, Wang W and Sui Y (2021). Path selection of a train of spherical capsules in a branched microchannel. Journal of Fluid Mechanics, Cambridge University Press (CUP) vol. 923 22-07-2021
Pang X, Li W, Chang L, Gautrot JE, Wang W and Azevedo HS (2021). Hyaluronan (HA) Immobilized on Surfaces via Self-Assembled Monolayers of HA-Binding Peptide Modulates Endothelial Cell Spreading and Migration through Focal Adhesion. ACS Applied Materials and Interfaces, American Chemical Society vol. 13 (22), 25792-25804. 26-05-2021
Wu Y, Fortunato GM, Okesola BO, Brocchetti FLPD, Suntornnond R, Connelly J, De Maria C, Rodriguez-Cabello JC, Vozzi G, Wang W and Mata A (2021). An interfacial self-assembling bioink for the manufacturing of capillary-like structures with tuneable and anisotropic permeability. Biofabrication, IOP Publishing vol. 13 (3) 08-04-2021
Pu JH, Wang SK, Sun J, Wang W and Wang HS (2021). Stable and Efficient Nanofilm Pure Evaporation on Nanopillar Surfaces. Langmuir vol. 37 (12), 3731-3739. 08-03-2021
Guo T, Wang Y, Jia J, Mao X, Stankiewicz E, Scandura G, Burke E, Xu L, Marzec J, Davies CR, Lu JJ, Rajan P, Grey A, Tipples K, Hines J, Kudahetti S, Oliver T, Powles T, Alifrangis C, Kohli M, et al. (2021). The Identification of Plasma Exosomal miR-423-3p as a Potential Predictive Biomarker for Prostate Cancer Castration-Resistance Development by Plasma Exosomal miRNA Sequencing. Frontiers in Cell and Developmental Biology, Frontiers vol. 8 07-01-2021
Lin T, Wang Z, Lu R, Wang W and Sui Y (2021). A high-throughput method to characterize membrane viscosity of flowing microcapsules. Physics of Fluids, AIP Publishing vol. 33 (1) 01-01-2021
Lin T, Wang Z, Wang W and Sui Y (2021). A neural network-based algorithm for high-throughput characterisation of viscoelastic properties of flowing microcapsules. Soft Matter, Royal Society of Chemistry (RSC) vol. 17 (15), 4027-4039. 01-01-2021
Lin T, Wang Z, Wang W and Sui Y (2021). Correction: A neural network-based algorithm for high-throughput characterisation of viscoelastic properties of flowing microcapsules. Soft Matter, Royal Society of Chemistry (RSC) vol. 17 (15), 4213-4213. 01-01-2021
Fu S, Meng H, Inamdar S, Das B, Gupta H, Wang W, Thompson C and Knight M (2021). Activation of TRPV4 by mechanical, osmotic or pharmaceutical stimulation is anti-inflammatory blocking IL-1β mediated articular cartilage matrix destruction., Editors: Block JA. Osteoarthritis and Cartilage, Elsevier 01-01-2021
Pu JH, Sheng Q, Sun J, Wang W and Wang HS (2021). Molecular Dynamics Simulation of Effect of Temperature Difference on Surface Condensation. Advances in Heat Transfer and Thermal Engineering 99-103. 01-01-2021
2020
He S, Yang F, Yang M, An W, Maguire EM, Chen Q, Xiao R, Wu W, Zhang L, Wang W and Xiao Q (2020). miR-214-3p-Sufu-GLI1 is a novel regulatory axis controlling inflammatory smooth muscle cell differentiation from stem cells and neointimal hyperplasia. Stem Cell Research & Therapy, Springer Nature vol. 11 (1) 03-11-2020
Wu Y, Wang W, Mata A, Pugno N, Azevedo H, Karabasov S and Titirici M-M (2020). Disordered protein-graphene oxide co-assembly and supramolecular biofabrication of functional fluidic devices. Nature Communications, Nature Research (part of Springer Nature) 04-03-2020
Pu JH, Sun J, Wang W and Wang HS (2020). Generation and Evolution of Nanobubbles on Heated Nanoparticles: A Molecular Dynamics Study. Langmuir: the ACS journal of surfaces and colloids, American Chemical Society vol. 36, 2375-2382. 03-02-2020
Yang J, Moraga A, Xu J, Zhao Y, Luo P, Lao KH, Margariti A, Zhao Q, Ding W, Wang G, Zhang M, Zheng L, Zhang Z, Hu Y, Wang W, Shen L, Smith A, Shah AM, Wang Q and Zeng L (2020). A histone deacetylase 7‐derived peptide promotes vascular regeneration via facilitating 14‐3‐3γ phosphorylation. Stem Cells, Oxford University Press (OUP) vol. 38 (4), 556-573. 29-01-2020
Fan B, Wang W, Wang T, Xie B, Zhang H, Liao Y, Lu C and Guo L (2020). Problematic Internet Use, Non-Medical Use of Prescription Drugs, and Depressive Symptoms among Adolescents: A Large-Scale Study in China. International Journal of Environmental Research and Public Health, MDPI vol. 17 (3) 26-01-2020
2019
Pu JH, Sun J, Sheng Q, Wang W and Wang HS (2019). Dependences of Formation and Transition of the Surface Condensation Mode on Wettability and Temperature Difference. Langmuir: the ACS journal of surfaces and colloids, American Chemical Society vol. 36, 456-464. 15-12-2019
Arokiasamy S, King R, Boulaghrasse H, Poston R, Nourshargh S, Wang W and Voisin M-B (2019). Heparanase-dependent Remodelling of Initial Lymphatic Glycocalyx 1 Regulates Tissue-fluid Drainage during Acute Inflammation in vivo. Frontiers in Immunology, Frontiers Media 04-10-2019
Li W and Wang W (2019). Membrane tension regulates syndecan-1 expression through actin remodelling. Biochim Biophys Acta Gen Subj vol. 1863 (11), 129413-129413. 08-08-2019
Pang X, Li W, Landwehr E, Yuan Y, Wang W and Azevedo HS (2019). Mimicking the endothelial glycocalyx through the supramolecular presentation of hyaluronan on patterned surfaces. Faraday Discuss 15-07-2019
Fu S, Thompson CL, Ali A, Wang W, Chapple JP, Mitchison HM, Beales PL, Wann AKT and Knight MM (2019). Mechanical loading inhibits cartilage inflammatory signalling via an HDAC6 and IFT-dependent mechanism regulating primary cilia elongation. Osteoarthritis and Cartilage, Elsevier vol. 27 (7), 1064-1074. 25-03-2019
PU JH, SHENG Q, Sun J, WANG W and WANG H (2019). Dependence of nano-confined surface condensation on tangentially external force field. Journal of Molecular Liquids, Elsevier 21-03-2019
Wang F, Contò FP, Naz N, Castrejón-Pita JR, Castrejón-Pita AA, Bailey CG, Wang W, Feng JJ and Sui Y (2019). A fate-alternating transitional regime in contracting liquid filaments. Journal of Fluid Mechanics vol. 860, 640-653. 10-02-2019
Lu Y and Wang W (2019). Biofluids: Microcirculation. Comprehensive Biotechnology 32-46. 01-01-2019
Wang Z, Sui Y, Wang W, Barthѐs-Biesel D and Salsac A-V (2019). Path Selection of a Spherical Capsule in a Branched Channel. Molecular & Cellular Biomechanics, Computers, Materials and Continua (Tech Science Press) vol. 16 (S2), 42-43. 01-01-2019
2018
Peng J, Wang W, Zheng L, Guan Y, Ye G, Tong H, Wang Y and Wang Q (2018). Serum Cystatin C Levels in Twin Pregnancy versus Singleton Pregnancy. Lab Medicine, Oxford University Press (OUP) vol. 50 (2), 163-167. 04-12-2018
Tang P, di Cio S, Wang W and E Gautrot J (2018). Surface-Initiated Poly(oligo(2-alkyl-2-oxazoline)methacrylate) Brushes. Langmuir vol. 34 (34), 10019-10027. 28-08-2018
Sheng Q, Sun J, You X, Wang W and Wang HS (2018). Detection of liquid-vapour interface in molecular dynamics simulation. 16th International Heat Transfer Conference. 10-08-2018
ZHAO T, DENG L, WANG W, Elson D and SU L (2018). Bayes’ theorem based binary algorithm for fast reference-less calibration of a multimode fiber. Optics Express, Optical Society of America (OSA) 06-08-2018
Wang Z, Sui Y, Salsac AV, Barthès-Biesel D and Wang W (2018). Path selection of a spherical capsule in a microfluidic branched channel: Towards the design of an enrichment device. Journal of Fluid Mechanics vol. 849, 136-162. 18-06-2018
Liu J, Zhang Y, Zhao Q, Situ B, Zhao J, Luo S, Li B, Yan X, Vadgama P, Su L, Ma W, Wang W and Zheng L (2018). Bifunctional aptamer-mediated catalytic hairpin assembly for the sensitive and homogenous detection of rare cancer cells. Anal Chim Acta vol. 1029, 58-64. 04-05-2018
Yang J, Xu J, Danniel M, Wang X, Wang W, Zeng L and Shen L (2018). The interaction between XBP1 and eNOS contributes to endothelial cell migration. Experimental Cell Research, Elsevier vol. 363 (2), 262-270. 17-01-2018
Zhao K, Zhao Q, Guo Z, Chen Z, Hu Y, Su J, Chen L, He Z, Cai X, Chen M, Zheng L, Wang W and Wang Q (2018). Hsa_Circ_0001275: A Potential Novel Diagnostic Biomarker for Postmenopausal Osteoporosis. Cellular Physiology and Biochemistry, Karger Publishers vol. 46 (6), 2508-2516. 01-01-2018
2017
SHENG Q, SUN J, WANG W, WANG HS and BAILEY CG (2017). How solid surface free energy determines coalescence-induced nanodroplet jumping: A molecular dynamics investigation. Journal of Applied Physics, AIP Publishing vol. 122, 245301 (2017) 27-12-2017
Hughes-Brittain NF, Qiu L, Wang W, Peijs T and Bastiaansen CWM (2017). Degradation and biocompatibility of photoembossed PLGA-acrylate blend for improved cell adhesion. J Biomed Mater Res B Appl Biomater vol. 106 (1), 163-171. 08-12-2017
Norton R, Honarbakshs S, Wang W, Schilling R and Hunter R (2017). 118A novel method to determine left atrial compliance in vivo: relationship with markers of remodelling in AF. EP Europace, Oxford University Press (OUP) vol. 19 (suppl_1), i49-i49. 01-10-2017
WANG W (2017). Effects of High Pressure on Phospholipid Bilayers. Journal of Physical Chemistry B, American Chemical Society 19-09-2017
Li W and Wang W (2017). Structural alteration of the endothelial glycocalyx: contribution of the actin cytoskeleton. Biomech Model Mechanobiol 14-08-2017
Hughes-Brittain NF, Qiu L, Picot OT, Wang W, Peijs T and Bastiaansen CWM (2017). Surface texturing of electrospun fibres by photoembossing using pulsed laser interference holography and its effects on endothelial cell adhesion. Polymer (United Kingdom) vol. 125, 40-49. 26-07-2017
Yu B, Kiechl S, Qi D, Wang X, Song Y, Weger S, Mayr A, Le Bras A, Karamariti E, Zhang Z, Barco Barrantes ID, Niehrs C, Schett G, Hu Y, Wang W, Willeit J, Qu A and Xu Q (2017). A Cytokine-Like Protein Dickkopf-Related Protein 3 Is Atheroprotective. Circulation vol. 136 (11), 1022-1036. 03-07-2017
Arokiasamy S, Nourshargh S, Wang W and Voisin M-B (2017). Characterisation of the lymphatic endothelium glycocalyx and its regulation during acute inflammation in vivo. NA. 01-06-2017
VOISIN M (2017). Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation. Scientific Reports, Nature Publishing Group 13-03-2017
2016
Qiu L, Hughes-Brittain NF, Bastiaansen CWM, Peijs T and Wang W (2016). Responses of Vascular Endothelial Cells to Photoembossed Topographies on Poly(Methyl Methacrylate) Films. J Funct Biomater vol. 7 (4) 09-12-2016
Wang Z, Sui Y, Salsac AV, Barthès-Biesel D and Wang W (2016). Motion of a spherical capsule in branched tube flow with finite inertia. Journal of Fluid Mechanics vol. 806, 603-626. 13-10-2016
Mo J, Prévost SF, Blowes LM, Egertová M, Terrill NJ, Wang W, Elphick MR and Gupta HS (2016). Interfibrillar stiffening of echinoderm mutable collagenous tissue demonstrated at the nanoscale. Proc Natl Acad Sci U S A 05-10-2016
Sheng Q, Sun J, Wang Q, Wang W and Wang HS (2016). On the onset of surface condensation: Formation and transition mechanisms of condensation mode. Scientific Reports, Nature Publishing Group: Open Access Journals - Option C 02-08-2016
Yu B, Wong MM, Potter CMF, Simpson RML, Karamariti E, Zhang Z, Zeng L, Warren D, Hu Y, Wang W and Xu Q (2016). Vascular Stem/Progenitor Cell Migration Induced by Smooth Muscle Cell-Derived Chemokine (C-C Motif) Ligand 2 and Chemokine (C-X-C motif) Ligand 1 Contributes to Neointima Formation. Stem Cells, Wiley vol. 34 (9), 2368-2380. 28-06-2016
Lv W, Zhao J, Situ B, Li B, Ma W, Liu J, Wu Z, Wang W, Yan X and Zheng L (2016). A target-triggered dual amplification strategy for sensitive detection of microRNA. Biosensors and Bioelectronics 19-04-2016
KNIGHT MM, wang W, wang Z, wann A, thompson C and hassen A (2016). IFT88 influences chondrocyte actin organization and biomechanics. Osteoarthritis and Cartilage 01-03-2016
Orsi M, Ding W, Palaiokostas M and Wang W (2016). Molecular Simulation of Lipids and Water: Atomistic, Coarse-Grained, and Mixed Resolutions. 01-02-2016
Ding W, Palaiokostas M, Wang W and Orsi M (2016). Atomistic and Coarse-Grained Molecular Simulations of Mixed Lamellar/Nonlamellar Lipid Membranes. 01-02-2016
2015
Ma W, Situ B, Lv W, Li B, Yin X, Vadgama P, Zheng L and Wang W (2015). Electrochemical determination of microRNAs based on isothermal strand-displacement polymerase reaction coupled with multienzyme functionalized magnetic micro-carriers. Biosensors and Bioelectronics vol. 80, 344-351. 21-12-2015
Ding W, Palaiokostas M, Wang W and Orsi M (2015). Effects of Lipid Composition on Bilayer Membranes Quantified by All-Atom Molecular Dynamics. The Journal of Physical Chemistry B, American Chemical Society (ACS) vol. 119 (49), 15263-15274. 24-11-2015
Zeng L, Li Y, Yang J, Wang G, Margariti A, Xiao Q, Zampetaki A, Yin X, Mayr M, Mori K, Wang W, Hu Y and Xu Q (2015). XBP 1-Deficiency Abrogates Neointimal Lesion of Injured Vessels Via Cross Talk With the PDGF Signaling. Arteriosclerosis Thrombosis and Vascular Biology, Wolters Kluwer vol. 35 (10), 2134-2144. 27-08-2015
Yu X, Zhang L, Wen G, Zhao H, Luong LA, Chen Q, Huang Y, Zhu J, Ye S, Xu Q, Wang W and Xiao Q (2015). Upregulated sirtuin 1 by miRNA-34a is required for smooth muscle cell differentiation from pluripotent stem cells. Cell Death and Differentiation vol. 22 (7), 1170-1180. 01-07-2015
Wang X, Karamariti E, Simpson R, Wang W and Xu Q (2015). Dickkopf Homolog 3 Induces Stem Cell Differentiation into Smooth Muscle Lineage via ATF6 Signalling*. Journal of Biological Chemistry, Elsevier vol. 290 (32), 19844-19852. 23-06-2015
Yang J, Zeng L, Wang W and Li Y (2015). Abstract 662: X-box Binding Protein 1 Splicing Promotes Wound Healing via Modulating Endothelial Nitric Oxide Synthase. Arteriosclerosis Thrombosis and Vascular Biology, Wolters Kluwer vol. 35 (suppl_1) 01-05-2015
Yu B, Wong MM, Wang W, Hu Y and Xu Q (2015). Abstract 200: Vascular Stem Cell Migration in Response to Monocyte Chemoattractant Protein-1 Promotes Neointima Lesion Formation. Arteriosclerosis Thrombosis and Vascular Biology, Wolters Kluwer vol. 35 (suppl_1) 01-05-2015
Yu B, Wong MM, Potter C, Hu Y, Wang W and Xu Q (2015). Abstract 9: Smooth Muscle Cells Are Crucial for Vascular Stem Cell Migration and Vasculogenesis via Keratinocyte Cell-derived Chemokine. Arteriosclerosis Thrombosis and Vascular Biology, Wolters Kluwer vol. 35 (suppl_1) 01-05-2015
Yang J-Y, Wang Q, Wang W and Zeng L-F (2015). Histone deacetylases and cardiovascular cell lineage commitment. World Journal of Stem Cells, Baishideng Publishing Group vol. 7 (5), 852-858. 01-01-2015
2014
Liu W, Wang X, Bai K, Lin M, Sukhorukov G and Wang W (2014). Microcapsules functionalized with neuraminidase can enter vascular endothelial cells in vitro. J R Soc Interface vol. 11 (101) 06-12-2014
Wang Z, Irianto J, Kazun S, Wang W and Knight MM (2014). The rate of hypo-osmotic challenge influences regulatory volume decrease (RVD) and mechanical properties of articular chondrocytes. Osteoarthritis and Cartilage, Elsevier vol. 23 (2), 289-299. 11-11-2014
Deo DI, Gautrot JE, Sukhorukov GB and Wang W (2014). Biofunctionalization of PEGylated microcapsules for exclusive binding to protein substrates. Biomacromolecules vol. 15 (7), 2555-2562. 14-07-2014
Sun J, Wang W and Wang HS (2014). Viscous dissipation effect in nano-confined shear flows: a comparative study between molecular dynamics and multi-scale hybrid simulations. Microfluidics and Nanofluidics, Springer Link 01-05-2014
Hughes-Brittain NF, Qiu L, Wang W, Peijs T and Bastiaansen CWM (2014). Photoembossing of surface relief structures in polymer films for biomedical applications. J Biomed Mater Res B Appl Biomater vol. 102 (2), 214-220. 01-02-2014
Bai K and Wang W (2014). Shear stress-induced redistribution of the glycocalyx on endothelial cells in vitro. Biomechanics and Modeling in Mechanobiology vol. 13 (2), 303-311. 01-01-2014
Lu$y.lu@derby.ac.uk$School of Engineering and Technology Y, University of Derby , Derby , Science UW-QLSOEAM, Mary , University of London , London , Oraifige$School of Engineering and Technology UI and Science UWWSOEAM (2014). Converging Parallel Plate Flow Chambers for Studies on the Effect of the Spatial Gradient of Wall Shear Stress on Endothelial Cells. Journal of Biosciences and Medicines, Scientific Research Publishing vol. 02 (02), 50-56. 01-01-2014
2013
Wang Z, Sui Y, Spelt PDM and Wang W (2013). Three-dimensional dynamics of oblate and prolate capsules in shear flow. Physical Review E: Statistical, Nonlinear, and Soft Matter Physics, American Physical Society vol. 88 26-11-2013
Zeng L, Wang G, Ummarino D, Margariti A, Xu Q, Xiao Q, Wang W, Zhang Z, Yin X, Mayr M, Cockerill G, Li JY-S, Chien S, Hu Y and Xu Q (2013). Histone deacetylase 3 unconventional splicing mediates endothelial-to-mesenchymal transition through transforming growth factor β2. J Biol Chem vol. 288 (44), 31853-31866. 01-11-2013
Luo Z, Wen G, Wang G, Pu X, Ye S, Xu Q, Wang W and Xiao Q (2013). MicroRNA-200C and -150 play an important role in endothelial cell differentiation and vasculogenesis by targeting transcription repressor ZEB1. Stem Cells vol. 31 (9), 1749-1762. 01-09-2013
Ye S, Wang W and Shao X (2013). Numerical simulation of the hydrodynamics of endothelial glycocalyx under shear flow. IEEE International Conference on Control and Automation, ICCA, 923-928. 26-08-2013
Zhao H, Huang Y, Yu X, Wen G, Wang W, Ye S and Xiao Q (2013). Abstract 004: Microrna-22 Regulates Smooth Muscle Cell Differentiation From Stem Cells By Targeting Methyl Cpg Binding Protein 2. Circulation Research, Wolters Kluwer vol. 113 (suppl_1) 01-08-2013
Zeng L, Xiao Q, Chen M, Margariti A, Martin D, Ivetic A, Xu H, Mason J, Wang W, Cockerill G, Mori K, Yi-Shuan Li J, Chien S, Hu Y and Xu Q (2013). Vascular endothelial cell growth-activated XBP1 splicing in endothelial cells is crucial for angiogenesis. Circulation vol. 127 (16), 1712-1722. 23-04-2013
Margariti A, Li H, Chen T, Martin D, Vizcay-Barrena G, Alam S, Karamariti E, Xiao Q, Zampetaki A, Zhang Z, Wang W, Jiang Z, Gao C, Ma B, Chen YG, Cockerill G, Hu Y, Xu Q and Zeng L (2013). XBP1 mRNA splicing triggers an autophagic response in endothelial cells through BECLIN-1 transcriptional activation. Journal of Biological Chemistry vol. 288 (2), 859-872. 11-01-2013
Sun J, Wang W and Wang HS (2013). Dependence of nanoconfined liquid behavior on boundary and bulk factors. Physical Review E: Statistical, Nonlinear, and Soft Matter Physics vol. 87 01-01-2013
Sun J, Wang W and Wang HS (2013). Dependence between velocity slip and temperature jump in shear flows. The Journal of Chemical Physics, American Institute of Physics vol. 138 (23) 01-01-2013
Bai K and Wang W (2013). Shear stress-induced redistribution of the glycocalyx on endothelial cells in vitro. Biomechanics and Modeling in Mechanobiology, 1-9. 01-01-2013
Yu X, Zhang L, Wen G, Zhao H, Huang Y, Zhu J, Xu Q, Ye S, Wang W and Xiao Q (2013). MicroRNA-34a Regulated Sirtuin 1 is Required for Smooth Muscle Cell Differentiation From Murine and Human Embryonic Stem Cells. 01-01-2013
2012
Bai K and Wang W (2012). Spatio-temporal development of the endothelial glycocalyx layer and its mechanical property in vitro. Journal of the Royal Society Interface vol. 9 (74), 2290-2298. 07-09-2012
Lu Y, Michel CC and Wang W (2012). Inert gas clearance from tissue by co-currently and counter-currently arranged microvessels. J Appl Physiol (1985) vol. 113 (3), 487-497. 01-08-2012
Tsai TN, Kirton JP, Campagnolo P, Zhang L, Xiao Q, Zhang Z, Wang W, Hu Y and Xu Q (2012). Contribution of stem cells to neointimal formation of decellularized vessel grafts in a novel mouse model. American Journal of Pathology vol. 181 (1), 362-373. 01-07-2012
Hunter RJ, Liu Y, Lu Y, Wang W and Schilling RJ (2012). Left atrial wall stress distribution and its relationship to electrophysiologic remodeling in persistent atrial fibrillation. Circulation: Arrhythmia and Electrophysiology vol. 5 (2), 351-360. 01-04-2012
Maeda E, Ye S, Wang W, Bader DL, Knight MM and Lee DA (2012). Gap junction permeability between tenocytes within tendon fascicles is suppressed by tensile loading. Biomechanics and Modeling in Mechanobiology vol. 11 (3-4), 439-447. 01-03-2012
Luo Z, Wen G, Wang G, Pu X, Wang W, Ye S, Xu Q and Xiao Q (2012). Microrna-200c and-150 Play a Role in Human Embryonic Stem Cell Differentiation Into Endothelial Cells and Blood Vessel Formation by Targeting Transcription Repressor Zebi. CIRCULATION vol. 126 (21) 01-01-2012
2011
Luo Z, Xiao Q, Wang W and Xu Q (2011). DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TOWARDS THE ENDOTHELIAL LINEAGE INVOLVES MICRORNAS. HEART vol. 97 (20), 4-4. 01-10-2011
Lu Y and Wang W (2011). Biofluids. Comprehensive Biotechnology, Second Edition 237-250. 09-09-2011
Zheng L, Liu JZ, Hu YW, Zhong TY, Xiong SL, Wang W and Wang Q (2011). Simulated Microgravity, Erythroid Differentiation, and the Expression of Transcription Factor GATA-1 in CD34(+) Cells. AVIAT SPACE ENVIR MD vol. 82 (5), 513-517. 01-05-2011
Vikhansky A and Wang W (2011). Taylor dispersion in finite-length capillaries. CHEM ENG SCI vol. 66 (4), 642-649. 15-02-2011
Cohen RD, Brown CL, Nickols C, Levey P, Boucher BJ, Greenwald SE and Wang W (2011). Inbuilt mechanisms for overcoming functional problems inherent in hepatic microlobular structure. Comput Math Methods Med vol. 2011 01-01-2011
Luo ZL, Wang G, Wang W, Xiao QZ and Xu QB (2011). Signalling pathways that regulate endothelial differentiation from stem cells. FRONT BIOSCI-LANDMRK vol. 16, 472-485. 01-01-2011
Lu Y and Wang W (2011). 5.19 Biofluids | Microcirculation. Comprehensive Biotechnology 237-250. 01-01-2011
Lu Y and Wang W (2011). 5.03 Biofluids: Microcirculation. Comprehensive Biotechnology 32-46. 01-01-2011
2010
Bai K, Huang Y, Jia X, Fan Y and Wang W (2010). Endothelium oriented differentiation of bone marrow mesenchymal stem cells under chemical and mechanical stimulations. Journal of Biomechanics vol. 43 (6), 1176-1181. 01-04-2010
Lu Y and Wang W (2010). Multiscale modeling of fluid and solute transport in soft tissues and microvessels. Journal of Multiscale Modeling vol. 2 (1-2), 127-145. 01-03-2010
Ye S, Maeda E, Knight M, Lee D, Bader D and Wang W (2010). A compartment model to evaluate the permeability of gap junctions between tenocytes in tendon fascicles. FASEB JOURNAL vol. 24 01-01-2010
Maeda E, Ye S, Knight M, Wang W, Bader D and Lee D (2010). 1P338 1J1450 Mechano-regulation of gap junction communications between tenocytes within isolated fascicles(Bioengineering,Oral Presentations,The 48th Annual Meeting of the Biophysical Society of Japan). Seibutsu Butsuri, Biophysical Society of Japan vol. 50 (supplement2) 01-01-2010
2009
Li W, Yang SYY, Hu ZF, Winslet MC, Wang W and Seifalian AM (2009). Growth factors enhance endothelial progenitor cell proliferation under high-glucose conditions. MED SCI MONITOR vol. 15 (12), BR357-BR363. 01-12-2009
Campbell JJ, Maeda E, Ye SJ, Wang W and Lee DA (2009). Osteogenic differentiation of human mesenchymal stem cells within a perfused bioreactor system. European Cells and Materials vol. 18 (SUPPL. 2) 01-07-2009
Zeng L, Zampetaki A, Margariti A, Pepe AE, Alam S, Martin D, Xiao Q, Wang W, Jin ZG, Cockerill G, Mori K, Li YSJ, Hu Y, Chien S and Xu Q (2009). Sustained activation of XBP1 splicing leads to endothelial apoptosis and atherosclerosis development in response to disturbed flow. Proceedings of the National Academy of Sciences of the United States of America vol. 106 (20), 8326-8331. 19-05-2009
Wen PH, Hon YC and Wang W (2009). Dynamic responses of shear flows over a deformable porous surface layer in a cylindrical tube. APPL MATH MODEL vol. 33 (1), 423-436. 01-01-2009
2008
Wang QQ, Ping BH, Xu QB and Wang W (2008). Rheological effects of blood in a nonplanar distal end-to-side anastomosis. Journal of Biomechanical Engineering vol. 130 (5) 01-10-2008
Lu Y and Wang W (2008). Interaction between the interstitial fluid and the extracellular matrix in confined indentation. Journal of Biomechanical Engineering vol. 130 (4) 01-08-2008
Wang W and Wen PH (2008). Interaction between micro-particles in Oseen flows by the method of fundamental solutions. ENG ANAL BOUND ELEM vol. 32 (4), 318-327. 01-04-2008
2007
Wang W (2007). Change in properties of the glycocalyx affects the shear rate and stress distribution on endothelial cells. J BIOMECH ENG-T ASME vol. 129 (3), 324-329. 01-06-2007
Wen PH, Aliabadi MH and Wang W (2007). Movement of a spherical cell in capillaries using a boundary element method. J BIOMECH vol. 40 (8), 1786-1793. 01-01-2007
2006
Yin XK, Mayr M, Xiao QZ, Wang W and Xu QB (2006). Proteomic analysis reveals higher demand for antioxidant protection in embryonic stem cell-derived smooth muscle cells. PROTEOMICS vol. 6 (24), 6437-6446. 01-12-2006
Kyriacou G, Vadgama P and Wang W (2006). Characterization of a laminar flow cell for the prevention of biosensor fouling. MED ENG PHYS vol. 28 (10), 989-998. 01-12-2006
Ye SJ, Zhu KQ and Wang W (2006). Laminar flow of micropolar fluid in rectangular microchannels. ACTA MECH SINICA vol. 22 (5), 403-408. 01-10-2006
Garland SW, Wang W and Ward SA (2006). Indices of electromyographic activity and the slow component of oxygen uptake kinetics during high-intensity knee-extension exercise in humans. EUR J APPL PHYSIOL vol. 97 (4), 413-423. 01-07-2006
Lu Y, Parker KH and Wang W (2006). Effects of osmotic pressure in the extracellular matrix on tissue deformation. PHILOS T R SOC A vol. 364 (1843), 1407-1422. 15-06-2006
Chen J, Lu X-Y and Wang W (2006). Non-Newtonian effects of blood flow on hemodynamics in distal vascular graft anastomoses. J Biomech vol. 39 (11), 1983-1995. 01-01-2006
Chen J, Lu XY and Wang W (2006). Non-Newtonian effects of blood flow on hemodynamics in distal vascular graft anastomoses. J BIOMECH vol. 39 (11), 1983-1995. 01-01-2006
Li W, Bader DL and Wang W (2006). In vitro measurement of local oxygen partial pressures in chondrocyte-agarose constructs. 01-01-2006
2005
Lu Y, Levick JR and Wang W (2005). Modelling the concentration polarisation of hyaluronan on the surface of the synovial lining of infused joints. Proceedings of the 2005 Summer Bioengineering Conference vol. 2005, 824-825. 01-12-2005
Wang W and Vadgama P (2005). Microelectrodes and biocompatible sensors for skin pO01-12-2005
Yin XK, Mayr M, Xiao QZ, Mayr U, Tarelli E, Wait R, Wang W and Xu QB (2005). Proteomic dataset of Sca-1(+) progenitor cells. PROTEOMICS vol. 5 (17), 4533-4545. 01-11-2005
Lu Y, Levick JR and Wang W (2005). The mechanism of synovial fluid retention in pressurized joint cavities. MICROCIRCULATION vol. 12 (7), 581-595. 01-10-2005
Wang W (2005). In vivo measurements and theoretical modelling of oxygen partial pressure in outer layers of human skin. European Cells and Materials vol. 10 (SUPPL.2) 01-07-2005
Lu Y and Wang W (2005). Effects of cyclic loads on transport of fluid and solutes in cell seeded constructs. European Cells and Materials vol. 10 (SUPPL.2) 01-07-2005
Lu YL, Levick JR and Wang W (2005). Concentration polarisation of hyaluronan on the synovial lining of infused joints. 04-03-2005
Wang W (2005). Oxygen partial pressure in outer layers of skin: Simulation using three-dimensional multilayered models. MICROCIRCULATION vol. 12 (2), 195-207. 01-03-2005
2004
Lu Y, Levick JR and Wang W (2004). Concentration polarization of hyaluronan on the surface of the synovial lining of infused joints. J PHYSIOL-LONDON vol. 561 (2), 559-573. 01-12-2004
Wang W and Vadgama P (2004). O-2 microsensors for minimally invasive tissue monitoring. J ROY SOC INTERFACE vol. 1 (1), 109-117. 22-11-2004
WANG W, Zhuang LX, Chen J and Lu XY (2004). Numerical Analysis of the non-Newtonian blood flow in the non-planar artery with bifurcation. Journal of Hydrodynamics, B 01-01-2004
Lu Y and Wang W (2004). Solute transport in porous medium under external loads. Proceedings of the ASME Heat Transfer/Fluids Engineering Summer Conference 2004, HT/FED 2004 vol. 4, 693-699. 01-01-2004
WANG W, Lu Y and Levick JR (2004). Concentration polarisation of hyaluronan on the surface of the synovial lining of infused joints. Journal of Physiology 01-01-2004
VADGAMA PM and Wang W (2004). O2 microsensors for minimally invasive tissue monitoring. J.R. Soc. Interface vol. 1, 109-117. 01-01-2004
Lu Y, Parker KH, Vadgama P, Bader DL and Wang W (2004). Osmotic pressure of large molecules in extracellular matrix under dynamic loading conditions., Editors: Zhuang F and Li JC. 01-01-2004
Bader DL, WANG W, Lu Y, Li ZY and Parker KH (2004). Interaction between fluid and solid i extracellular matrix. Recent Advances in Fluid Mechanics, 679-682. 01-01-2004
Li ZY, Lu YL, Parker KH, Bader DL and Wang W (2004). Interaction between fluid and solid in extracellular matrix., Editors: Zhuang F and Li JC. 01-01-2004
2003
Wang W, Winlove CP and Michel CC (2003). Oxygen partial pressure in outer layers of skin of human finger nail folds. J PHYSIOL-LONDON vol. 549 (3), 855-863. 15-06-2003
2002
Chen W, Lu XY, Zhuang LX and Wang W (2002). A numerical study on oxygen transport by cutaneous microcirculation in outer layers of skin. Chinese Journal of Biomedical Engineering vol. 21 (6), 481-492. 20-12-2002
Watkins NV, Caro CG and Wang W (2002). Parallel-plate flow chamber for studies of 3D flow-endothelium interaction. BIORHEOLOGY vol. 39 (3-4), 337-342. 01-01-2002
Lu YL, Lu XY, Zhuang LX and Wang W (2002). Breaking symmetry in non-planar bifurcations: Distribution of flow and wall shear stress. BIORHEOLOGY vol. 39 (3-4), 431-436. 01-01-2002
WANG W, Lu YL, Zhuang LX and Lu XY (2002). Breaking symmetry in a non-planar bifurcations: distribution of flow and wall shear stress. Biorheology vol. 39, 431-436. 01-01-2002
WANG W, Lu XY, Chen W and Zhuang LX (2002). A numerical study on oxygen transport by cutaneous microcirculation in outer layers of skin. Journal of Biomedical Engineering vol. 21 (6), 216-228. 01-01-2002
2001
WANG W, Michel CC and Winlove CP (2001). Distribution of oxygen partial pressure in dermal papillae of human finger nailfolds. Journal of Vascular Research vol. 38 (4), 398-412. 01-01-2001
WANG W (2001). exchange mechanism between microvessels and interstitium in the renal medulla. Recent Advances in Biomechanics, 248-262. 01-01-2001
WANG W, Caro CG, Watkins NV and Gao ZX (2001). Parallel-plate flow chamber for studies of 3D flow-endothelium interaction. Journal of Physiology vol. 531P, 14-14. 01-01-2001
2000
Wang W and Michel CC (2000). Modeling exchange of plasma proteins between microcirculation and interstitium of the renal medulla. AM J PHYSIOL-RENAL vol. 279 (2), F334-F344. 01-08-2000
Wang W (2000). A critical parameter for transcapillary exchange of small solutes in countercurrent systems. J BIOMECH vol. 33 (5), 543-548. 01-05-2000
Wang W and Michel CC (2000). Clearance of plasma proteins from the renal medulla. FASEB J vol. 14 (4), A23-A23. 15-03-2000
1999
Wang W, Michel CC and Winlove CP (1999). A three-dimensional model on PO2 distribution in outer layers of human skin. J VASC RES vol. 36 (4), 333-333. 01-07-1999
Wang W, Michel CC and Winlove CP (1999). Modelling PO2 distribution in outer layers of skin. FASEB J vol. 13 (4), A25-A25. 12-03-1999
1998
Wang W and Parker KH (1998). Movement of spherical particles in capillaries using a boundary singularity method. J BIOMECH vol. 31 (4), 347-354. 01-04-1998
1997
Wang W and Michel CC (1997). Effects of anastomoses on salute transcapillary exchange in countercurrent systems. MICROCIRCULATION-LONDON vol. 4 (3), 381-390. 01-01-1997
1996
Wang W and Davies GA (1996). CFD studies of separation of mists from gases using vane-type separators. CHEMICAL ENGINEERING RESEARCH & DESIGN vol. 74 (A2), 232-238. 01-01-1996
1995
WANG W and MICHEL CC (1995). A SIMPLIFIED MATHEMATICAL-MODEL FOR THE TRANSCAPILLARY EXCHANGE OF FLUID AND ALBUMIN IN THE RENAL MEDULLA. J PHYSIOL-LONDON vol. 483P, P139-P139. 01-02-1995
WANG W and PARKER KH (1995). THE EFFECT OF DEFORMABLE POROUS SURFACE-LAYERS ON THE MOTION OF A SPHERE IN A NARROW CYLINDRICAL TUBE. J FLUID MECH vol. 283, 287-305. 25-01-1995
Wang W, Jia X and Davies GA (1995). A theoretical study of transient cross-flow filtration using force balance analysis. The Chemical Engineering Journal and The Biochemical Engineering Journal vol. 60 (1-3), 55-62. 01-01-1995
1994
PHILLIPS CG, PARKER KH and WANG W (1994). A MODEL FOR FLOW-THROUGH DISCONTINUITIES IN THE TIGHT JUNCTION OF THE ENDOTHELIAL INTERCELLULAR CLEFT. B MATH BIOL vol. 56 (4), 723-741. 01-07-1994
1993
ADAMSON RH, MICHEL CC, PARKER KH, PHILLIPS CG and WANG W (1993). PATHWAYS THROUGH THE INTERCELLULAR CLEFTS OF FROG MESENTERIC CAPILLARIES. J PHYSIOL-LONDON vol. 466, 303-327. 01-07-1993
Grants
Epithelial Exchange Surfaces – From organizing principles to novel culture models of the gatekeepers of the body - MSCA-2022-DN Martin KnightMartin Knight, Hazel Screen, Wen Wang, Clare Thompson and Cleo Bishop
£260,676 EPSRC - EU Scheme (01-12-2023 - 30-11-2027)
EPSRC Core Equipment Award 2022Wen Wang, Isabelle Mareschal, Massimo Poesio and Michael Reece
£875,000 EPSRC Engineering and Physical Sciences Research Council (03-01-2023 - 02-07-2024)
EPSRC Core Equipment 2020Wen Wang, Ketao Zhang, Matteo Palma and Ildar Farkhatdinov
£497,496 EPSRC Engineering and Physical Sciences Research Council (01-11-2020 - 30-04-2022)