Professor of Cell and Molecular Biology
Cell division, Cytoskeleton, Genomic Instability, Proteomics, Genomic variants, Cancer cell biology
Publications of specific relevance to the Centre for Molecular Cell BiologyMulti-SpinX: An advanced framework for automated tracking of mitotic spindles and kinetochores in multicellular environmentsSearch for chromosomal instability aiding variants reveal naturally occurring kinetochore gene variants that perturb chromosome segregationOpportunities and challenges for deep learning in cell dynamics research
Deep learning techniques and mathematical modeling allow 3D analysis of mitotic spindle dynamicsCounteraction between Astrin-PP1 and Cyclin-B-CDK1 pathways protects chromosome-microtubule attachments independent of biorientationElectrically tunable lenses - Eliminating mechanical axial movements during high-speed 3D live imagingMultinucleation associated DNA damage blocks proliferation in p53-compromised cellsKinetochores attached to microtubule-ends are stabilised by astrin bound pp1 to ensure proper chromosome segregationMARK2/Par1b kinase present at centrosomes and retraction fibres corrects spindle off-centring induced by actin disassemblySpindle rotation in human cells is reliant on a MARK2-mediated equatorial spindle centering mechanismProper rotation of the mitotic spindle requires an equatorial spindle centering mechanism in human cells.Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells.How are Dynamic Microtubules Stably Tethered to Human Chromosomes?Outer kinetochore phosphatases-PP2A-B56 and PP1-differently contribute to the establishment of chromosome-microtubule attachments.Kindlin1 regulates microtubule function to ensure normal mitosisEditorial: Quantitative Biology: Dynamics of Living Systems.A Semi-Supervised Approach for Refining Transcriptional Signatures of Drug Response and Repositioning PredictionsModeling of Noisy Spindle Dynamics Reveals Separable Contributions to Achieving Correct OrientationA proteomic study of mitotic phase-specific interactors of EB1 reveals a role for SXIP-mediated protein interactions in anaphase onsetPAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repairHigh-speed microscopy with an electrically tunable lens to image the dynamics of in vivo molecular complexesNeed for multi-scale systems to identify spindle orientation regulators relevant to tissue disorganization in solid cancersTAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomesLateral to End-on Conversion of Chromosome-Microtubule Attachment Requires Kinesins CENP-E and MCAK.Role of phosphatases in chromosome segregationLateral to End-on Conversion of Chromosome-Microtubule Attachment Requires Kinesins CENP-E and MCAKAutomated tracking of mitotic spindle pole positions shows that LGN is required for spindle rotation but not orientation maintenanceLateral to End-on Conversion of Chromosome-Microtubule Attachment Requires Kinesins CENP-E and MCAKMeeting report of the International Workshop on Quantitative Biology 2012: mesoscopic and microscopic worlds meetMicrotubule plus-ends within a mitotic cell are ‘moving platforms’ with anchoring, signalling and force-coupling rolesTAO1 maintains genome integrity by mediating the timely maturation of chromosome attachments made to lateral walls of microtubules.Microtubule-end bound complexes power chromosome movement during chromosome segregationA mechanism linking microtubule destabilisation to maturation of chromosome-microtubule attachmentsRoles for the Conserved Spc105p/Kre28p Complex in Kinetochore-Microtubule Binding and the Spindle Assembly CheckpointErratum To: The human kinetochore proteins Nnf1R and Mcm21R are required for accurate chromosome segregationStudying chromosome instability in the mouseAnaphase initiation is regulated by antagonistic ubiquitination and deubiquitination activitiesA functional genomic screen identifies a role for TAO1 kinase in spindle-checkpoint signallingAnaphase initiation is regulated by antagonistic ubiquitination and deubiquitination activitiesHsp90 is required to localise cyclin B and Msps/ch-TOG to the mitotic spindle in Drosophila and humansThe human kinetochore proteins Nnf1R and Mcm21R are required for accurate chromosome segregationMisorientation and reduced stretching of aligned sister kinetochores promote chromosome missegregation in EB1‐ or APC‐depleted cellsFunctional Genomic Screen Reveals the Role of a Novel Checkpoint Kinase, TAO1 in Mitosis and Cell Cycle ProgressionTiming and Checkpoints in the Regulation of Mitotic ProgressionChromosome segregation and genomic stabilityThe ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cellsThe Localization of Human Cyclins B1 and B2 Determines Cdk1 Substrate Specificity and Neither Enzyme Requires Mek to Disassemble the Golgi ApparatusPleiotropic effects of human papillomavirus type 16 E6 oncogene expression in human epithelial cell lines.Cytokeratin patterns of expression in human epithelial cell lines correlate with transcriptional activity of the human papillomavirus type 16 upstream regulatory region.Grants of specific relevance to the Centre for Molecular Cell BiologyDevelopment of AI framework for iPSC image analysis and integration with transcriptomics Cooperativity and forces in molecular interactions governing chromosome stabilityHow are mono-oriented chromosome-microtubule attachments protected to prevent errors in mitosis and associated cellular ageing?Joint-PhD Studentship with Carl ZeissHarmonised IAA grant - Improve and accelerate the impact of QMUL research BBSRCKTP Capacity Building Competition 2022Highthroughput Light Sheet Microscopy: Imaging Across ScalesA confocal microscope for multidisciplinary dynamic studies of complex biological systemsThe Lexicon of miRISC: Deconstructing the functional complexity of the miRNA induced silencing complexKTP Project with Zeiss- Generalisation of SpinX software (Deep Learning based system and 3D modellingUK-China partnership: Chromosomal Instability aiding Genetic Variants (CIVa) linked to human ageingSuper-Resolution Microscopy of live cells in 3DProtecting Chromosome Number: how cells establish, monitor and maintain chromosome-microtubule interaction?Professor Thomas Iskratsch, Professor in Cardiovascular Mechanobiology and Bioengineering, has been awarded a highly coveted Programme Grant of £1.2 million from the British Heart Foundation (BHF). This significant funding will support groundbreaking research into how mechanical forces contribute to cardiovascular disease, a leading cause of mortality worldwide. Professor Iskratsch's research aims ... [more]
